双光子探针用于高尔基体：双光子显微镜在活组织中检测高尔基体。
Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy.
机构信息
KU-KIST Graduate School of Converging Science and Technology , Korea University , 145 Anam-ro , Seongbuk-gu, Seoul 02841 , Republic of Korea.
Department of Chemistry , Daejin University , 1007 Hoguk-ro , Pocheon-si , Gyeonggi-do 11159 , Republic of Korea.
出版信息
Anal Chem. 2019 May 21;91(10):6669-6674. doi: 10.1021/acs.analchem.9b00607. Epub 2019 Apr 5.
We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[ d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[ d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10 cm·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.
我们从 6-(苯并[d]恶唑-2-基)-2-萘胺和 2,5-双(苯并[d]恶唑-2-基)吡嗪衍生物出发，开发了两种发射蓝、黄光的双光子探针（BGolgi-blue 和 PGolgi-yellow），将其作为荧光团，将跨高尔基网络肽（SDYQRL）作为高尔基器靶向部分。用 BGolgi-blue 和 PGolgi-yellow 标记的 HeLa 细胞分别发射出 462nm 和 560nm 的双光子激发荧光，其有效双光子作用截面值分别为 1860 和 1600×10 cm·s/photon。这些探针可通过双光子显微镜在广泛分离的波长区域内，以高选择性和最小的 pH 干扰检测活细胞和活组织内部的高尔基器，并且具有光稳定性和低细胞毒性。
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