a Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Sciences , South China Normal University , Guangzhou , P. R. China.
b International Business College , South China Normal University , Guangzhou , P. R. China.
Int J Phytoremediation. 2019;21(9):857-865. doi: 10.1080/15226514.2019.1577355. Epub 2019 Mar 28.
Little attention has been paid to the combined use of arbuscular mycorrhizal fungus (AMF) and steel slag (SS) for ameliorating heavy metal polluted soils. A greenhouse pot experiment was conducted to study the effects of SS and AMF- (Fm), (Gv) and (Ri) on plant growth and Cd, Pb uptake by maize grown in soils added with 5 mg Cd kg and 300 mg Pb kg soil. The combined usage of AMF and SS (AMF + SS) promoted maize growth, and Gv + SS had the most obvious effect. Meanwhile, single SS addition and AMF + SS decreased Cd, Pb concentrations in maize, and the greater reductions were found in combined utilization, and the lowest Cd, Pb concentrations of maize appeared in Gv + SS. Single SS amendment and AMF + SS enhanced soil pH and decreased soil diethylenetriaminepentaacetic acid (DTPA)-extractable Cd, Pb concentrations. Furthermore, alone and combined usage of AMF and SS increased contents of soil total glomalin. Our research indicated a synergistic effect between AMF and SS on enhancing plant growth and reducing Cd, Pb accumulation in maize, and Gv + SS exerted the most pronounced effect. This work suggests that AMF inoculation in combination with SS addition may be a potential method for not only phytostabilization of Pb-Cd-contaminated soil but maize safety production.
人们对丛枝菌根真菌（AMF）和钢渣（SS）联合用于改良重金属污染土壤的关注较少。本温室盆栽试验研究了 SS 和 AMF-（Fm）、（Gv）和（Ri）对添加 5mg Cd kg 和 300mg Pb kg 土壤的玉米在土壤中生长和 Cd、Pb 吸收的影响。AMF 和 SS 的联合使用（AMF+SS）促进了玉米的生长，而 Gv+SS 的效果最明显。同时，单一 SS 加和 AMF+SS 降低了玉米中的 Cd、Pb 浓度，联合利用的降低效果更为明显，而 Gv+SS 中玉米的 Cd、Pb 浓度最低。单一 SS 处理和 AMF+SS 提高了土壤 pH 值，降低了土壤二乙烯三胺五乙酸（DTPA）可提取的 Cd、Pb 浓度。此外，AMF 单独和联合使用增加了土壤总 Glomalin 的含量。我们的研究表明，AMF 和 SS 之间存在协同作用，可增强植物生长，降低玉米对 Cd、Pb 的积累，而 Gv+SS 的效果最为显著。这项工作表明，接种 AMF 结合 SS 添加可能是一种潜在的方法，不仅可以用于稳定 Pb-Cd 污染土壤，还可以保证玉米的安全生产。
