Bandyopadhyay Sulalit, Sharma Anuvansh, Glomm Wilhelm Robert
Ugelstad Laboratory, Department of Chemical Engineering, Norwegian University of Science and technology (NTNU), N-7491 Trondheim, Norway.
Polymer Particle and Surface Chemistry Research Group, SINTEF Materials and Chemistry, N-7465 Trondheim, Norway.
Gels. 2017 Nov 8;3(4):42. doi: 10.3390/gels3040042.
Here, we report the synthesis and functionalization of five different shapes of Au nanoparticles (NPs), namely nanorods, tetrahexahedral, bipyramids, nanomakura, and spheres with PEG and poly (-isopropylacrylamide)-acrylic acid (pNIPAm-AAc) hydrogels. The anisotropic NPs are synthesized using seed-mediated growth in the presence of silver. The NPs have been characterized using Dynamic Light Scattering (DLS), zeta potential measurements, UV-Visible spectrophotometry (UV-Vis), and Scanning Transmission Electron Microscopy (S(T)EM). Cyt was loaded into the PEG-hydrogel-coated AuNPs using a modified breathing-in method. Loading efficiencies (up to 80%), dependent on particle geometry, concentration, and hydrogel content, were obtained. Release experiments conducted at high temperature (40 °C) and acidic pH (3) showed higher release for larger sizes of PEG-hydrogel-coated AuNPs, with temporal transition from spherical to thin film release geometry. AuNP shape, size, number density, and hydrogel content are found to influence the loading as well as release kinetics of Cyt from these systems.
在此，我们报告了五种不同形状的金纳米颗粒（NPs）的合成与功能化，即纳米棒、四面体、双锥体、纳米枕以及带有聚乙二醇（PEG）和聚（N - 异丙基丙烯酰胺）- 丙烯酸（pNIPAm - AAc）水凝胶的球体。各向异性的纳米颗粒是在银存在的情况下通过种子介导生长法合成的。这些纳米颗粒已通过动态光散射（DLS）、zeta 电位测量、紫外可见分光光度法（UV - Vis）以及扫描透射电子显微镜（S(T)EM）进行了表征。使用改良的吸入法将细胞色素 c（Cyt ）载入 PEG 水凝胶包覆的金纳米颗粒中。获得了取决于颗粒几何形状、浓度和水凝胶含量的载入效率（高达 80%）。在高温（40°C）和酸性 pH（3）条件下进行的释放实验表明，较大尺寸的 PEG 水凝胶包覆的金纳米颗粒释放量更高，释放几何形状随时间从球形转变为薄膜形。发现金纳米颗粒的形状、尺寸、数量密度和水凝胶含量会影响 Cyt 从这些体系中的载入以及释放动力学。
