Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing 210096 Jiangsu, PR China.
Biosens Bioelectron. 2012 Oct-Dec;38(1):94-9. doi: 10.1016/j.bios.2012.05.005. Epub 2012 May 16.
An immunoassay based on surface enhanced Raman scattering (SERS) has been developed using immuno-gold/silver core-shell nanorods with a high sensitivity. The features of these nanoparticles were characterized by UV-vis extinction spectra, TEM images, EDX analyses and SERS spectra. It was found that the obtained gold/silver core-shell nanorods showed a much higher SERS activity than uncoated gold nanorods. After the gold/silver core-shell nanorods were modified with antibody and employed in immunoassay, the antigen concentration-dependent SERS spectra and dose-response calibration curves were obtained. By comparison, it can be concluded that the detection limit of gold/silver core-shell nanorods based immunoassay reaches 70 fM, which is 10(4) times lower than gold nanorods based detection. As a result, SERS probes fabricated with gold/silver core-shell nanorods are demonstrated to be advantageous to those synthesized with gold nanorods due to their highly increased sensitivity in sandwich immunoassay, which also indicates the potential use of these gold/silver core-shell nanorods in other biological sensing applications.
基于表面增强拉曼散射（SERS）的免疫分析已经开发出来，使用具有高灵敏度的免疫金/银核壳纳米棒。这些纳米粒子的特征通过紫外-可见消光光谱、TEM 图像、EDX 分析和 SERS 光谱进行了表征。结果表明，所得到的金/银核壳纳米棒比未涂层的金纳米棒表现出更高的 SERS 活性。在金/银核壳纳米棒经过抗体修饰并应用于免疫分析后，获得了抗原浓度依赖的 SERS 光谱和剂量响应校准曲线。相比之下，可以得出结论，基于金/银核壳纳米棒的免疫分析的检测限达到 70fM，比基于金纳米棒的检测低 10(4)倍。因此，由于其在夹心免疫分析中高度提高的灵敏度，用金/银核壳纳米棒制备的 SERS 探针比用金纳米棒合成的探针更有利，这也表明这些金/银核壳纳米棒在其他生物传感应用中的潜在用途。
