Almond N M, Parkhouse R M
Parasite Immunol. 1986 Jul;8(4):391-406. doi: 10.1111/j.1365-3024.1986.tb00855.x.
A comparison of the humoral response to resistant (NIH) and susceptible (C3H) strains of mice, which reject adult worms at different rates during a primary infection, was made following infection with Trichinella spiralis. The serum concentration of immunoglobulins of the heavy chain classes IgM, IgG1, IgG2, IgG3 and IgA were determined by single radial immunodiffusion. Antibodies of the same immunoglobulin isotypes to biochemically defined, stage specific surface and secreted components of three stages of parasite development were also determined using an isotype specific immuno-coprecipitation assay. Independent variation of the responses of each immunoglobulin isotype was observed. The specific anti-parasite response did not reflect total serum immunoglobulin levels in all immunoglobulin classes, and this is discussed in relation to basic mechanisms of immunoglobulin class switching. Finally a close correlation was observed in resistant (NIH) mice between the production of IgA antibody to surface components of adult worms and accelerated expulsion of this stage of the worm from the gastrointestinal tract. The possible relevance of this IgA response is further indicated by the failure of susceptible mice to synthesise IgA antibodies to the same surface antigens.
在用旋毛虫感染小鼠后，对在初次感染期间以不同速率排斥成虫的抗性（NIH）和易感（C3H）品系小鼠的体液免疫反应进行了比较。通过单向放射免疫扩散法测定了重链类别为IgM、IgG1、IgG2、IgG3和IgA的免疫球蛋白的血清浓度。还使用同型特异性免疫共沉淀测定法测定了针对寄生虫发育三个阶段的生化定义的、阶段特异性表面和分泌成分的相同免疫球蛋白同种型的抗体。观察到每种免疫球蛋白同种型反应的独立变化。特异性抗寄生虫反应并未反映所有免疫球蛋白类别的总血清免疫球蛋白水平，并结合免疫球蛋白类别转换的基本机制进行了讨论。最后，在抗性（NIH）小鼠中观察到，针对成虫表面成分的IgA抗体产生与该阶段蠕虫从胃肠道的加速排出之间存在密切相关性。易感小鼠无法合成针对相同表面抗原的IgA抗体，这进一步表明了这种IgA反应的潜在相关性。
