Robbins Ruth N, Bergdoll Merlin S
Food Research Institute and Department of Food Microbiology and Toxicology, University of Wisconsin-Madison, 1925 Willow Drive, Madison, Wisconsin 53706.
J Food Prot. 1984 Mar;47(3):172-176. doi: 10.4315/0362-028X-47.3.172.
An immunization method for production of antiserum to the staphylococcal enterotoxins in rabbits is presented. The bleeding schedule is tailored to the enterotoxin type. About 0.5 mg of staphylococcal enterotoxin is used per rabbit and serum harvest begins 11 weeks after the initial injection. Proposed are subcutaneous injections of 1, 3, 10, 20 and 30 μg of toxin with Freund's adjuvant on days 0, 3, 8, 24 and 28, respectively; and five weeks later, injections of 50, 100 and 300 μg on days 0, 3 and 8, respectively. Serum harvest ranges from a 4-week period for enterotoxin A to 8 or more weeks for enterotoxin E. Immunizations with all toxin types using the proposed or similar injection programs produced antibody titers from about 20 to over 100. Individual variation in response of rabbits in the same group was generally 3- to 5-fold, and in extreme instances, 10-fold. Immunization experiments were augmented by booster experiments in which the rabbit variable was held relatively constant by sequentially testing different schedules and doses on the same group of animals.
本文介绍了一种在兔子体内产生抗葡萄球菌肠毒素抗血清的免疫方法。采血计划根据肠毒素类型进行调整。每只兔子使用约0.5毫克葡萄球菌肠毒素，初次注射11周后开始采集血清。建议分别在第0、3、8、24和28天皮下注射1、3、10、20和30微克毒素与弗氏佐剂；五周后，分别在第0、3和8天注射50、100和300微克。血清采集时间从肠毒素A的4周不等，到肠毒素E的8周或更长时间。使用建议的或类似的注射方案对所有毒素类型进行免疫，产生的抗体效价约为20至超过100。同一组兔子的个体反应差异通常为3至5倍，在极端情况下为10倍。免疫实验通过加强实验得到补充，在加强实验中，通过在同一组动物上依次测试不同的方案和剂量，使兔子变量保持相对恒定。
