Dong Chen-Lu, Liu Xiao-Han, Wu Lin
China Medical University School & Hospital of Stomatology, Shenyang 110000, Liaoning, China.
China Medical University School & Hospital of Stomatology, Shenyang 110000, Liaoning, China;
Zhongguo Gu Shang. 2019 Mar 25;32(3):288-292. doi: 10.3969/j.issn.1003-0034.2019.03.018.
Supercritical large area or large segmental bone defects are still a clinical problem. Researchers are committed to the development of artificial bone materials, but in order to solve the problem of poor bone formation of artificial bone materials, people are paying more and more attention to application of bone marrow mesenchymal stem cells in bone tissue engineering. In this review, bone marrow mesenchymal stem cells, osteogenic differentiation, osteoblasts cells, clinical application were used as keywords to search CNKI database, Wanfang database, Weipu database and PUBMED database by computer. The isolation and culture of bone marrow mesenchymal stem cells, bone marrow mesenchymal stem cell identification method, osteogenic induction method, osteogenic differentiation identification and clinical applicationt were comprehensively summarized in order to provide a theoretical basis for its use as a seed cell in the treatment of bone tissue diseases. Scholars have preliminarily studied the treatment of bone and cartilage defects, osteoarthritis, femoral head necrosis and other diseases with bone marrow mesenchymal stem cells combined with transplantation, and obtained good clinical efficacy.However, bone marrow mesenchymal stem cells have certain advantages and disadvantages, and further clinical studies and long-term efficacy verification are needed.
超大面积或大段骨缺损仍是一个临床难题。研究人员致力于人工骨材料的研发，但为了解决人工骨材料骨形成不良的问题，人们越来越关注骨髓间充质干细胞在骨组织工程中的应用。在本综述中，以骨髓间充质干细胞、成骨分化、成骨细胞、临床应用为关键词，通过计算机检索中国知网数据库、万方数据库、维普数据库和PUBMED数据库。对骨髓间充质干细胞的分离培养、骨髓间充质干细胞鉴定方法、成骨诱导方法、成骨分化鉴定及临床应用进行了全面总结，以期为其作为治疗骨组织疾病的种子细胞提供理论依据。学者们已初步研究了骨髓间充质干细胞联合移植治疗骨与软骨缺损、骨关节炎、股骨头坏死等疾病，并取得了良好的临床疗效。然而，骨髓间充质干细胞有一定的优缺点，尚需进一步的临床研究及长期疗效验证。
