Yasuda M, Good R A, Day N K
Prep Biochem. 1986;16(3):217-26. doi: 10.1080/00327488608062467.
Feline interferon (IFN) was produced from spleen cells stimulated by Staphylococcus enterotoxin A (SEA). The IFN was purified by a three-step procedure using controlled pore glass adsorption chromatography, concanavalin A (ConA)-agarose column chromatography and gel filtration. The final product of these procedures which had activity of an IFN appeared as a single peak of activity with molecular weight of approximately 50,000. It was sensitive to acid and heat, suggesting that the isolated material was a gamma IFN. The total recovery of feline gamma IFN was 8.2%. Specific activity was 2.95 X 10(4) unit/micrograms protein and was concentrated 2.8 X 10(4) times. This preparation of purified feline gamma IFN was destroyed completely by 0.1% sodium dodecyl sulfate within 20 min. As an inducer of feline gamma IFN, SEA appeared to produce a more uniform IFN product than did ConA. Further, the presence of 10% ethylene glycol in the sample applied to ConA-agarose column as well as its absence in the elution buffer was effective in reducing contaminating acid- and heat-resistant IFNs in the preparation.
猫干扰素（IFN）由受A群链球菌肠毒素（SEA）刺激的脾细胞产生。该干扰素通过三步法进行纯化，即使用可控孔径玻璃吸附色谱法、伴刀豆球蛋白A（ConA）-琼脂糖柱色谱法和凝胶过滤法。这些步骤的最终产物具有干扰素活性，表现为单一活性峰，分子量约为50,000。它对酸和热敏感，表明分离出的物质是γ干扰素。猫γ干扰素的总回收率为8.2%。比活性为2.95×10⁴单位/微克蛋白质，浓缩了2.8×10⁴倍。这种纯化的猫γ干扰素制剂在20分钟内被0.1%的十二烷基硫酸钠完全破坏。作为猫γ干扰素的诱导剂，SEA似乎比ConA产生的干扰素产物更均匀。此外，应用于ConA-琼脂糖柱的样品中存在10%的乙二醇以及洗脱缓冲液中不存在乙二醇，有效地减少了制剂中污染的耐酸和耐热干扰素。
