Department of Zoology, Mizoram University, Aizawl, Mizoram, 796 004, India.
Department of Zoology, Mizoram University, Aizawl, Mizoram, 796 004, India.
J Steroid Biochem Mol Biol. 2019 Jun;190:64-75. doi: 10.1016/j.jsbmb.2019.03.016. Epub 2019 Mar 25.
Testicular aging leads to a decrease in spermatogenesis and steroidogenesis. Vitamin D plays an important role in reproduction by modulating testicular steroidogenesis. As the role of vitamin D3 in testicular steroidogenesis during aging has not been explored, the aim of this study was to evaluate the effects of vitamin D3 on testicular functions in d-gal-induced aged rats. Vitamin D3 treatment on d-gal-induced aged rats resulted in significant improvement in sperm parameters, histoarchitecture, serum testosterone, and rostenedione and estrogen levels. The results of both in vivo and in vitro studies showed that vitamin D3 directly regulates testicular steroidogenic markers. Vitamin D3 treatment also increased CYP19A1 and decreased AR expression in the testes of d-gal-induced aged and normal rats. These results suggest that estrogen-mediated action may be responsible for an improvement in spermatogenesis in aged testis. Furthermore, it may be suggested vitamin D3 has a protective role in the aged testis and unaffected spermatogenesis in normal rats treated with vitamin D3 could be due to a balance between estrogen and androgen action.
睾丸老化导致精子发生和类固醇生成减少。维生素 D 通过调节睾丸类固醇生成在生殖中发挥重要作用。由于维生素 D3 在老化过程中对睾丸类固醇生成的作用尚未得到探索，本研究旨在评估维生素 D3 对 d-半乳糖诱导的老年大鼠睾丸功能的影响。维生素 D3 治疗 d-半乳糖诱导的老年大鼠可显著改善精子参数、组织形态、血清睾酮、rostenedione 和雌激素水平。体内和体外研究的结果均表明，维生素 D3 可直接调节睾丸类固醇生成标记物。维生素 D3 治疗还增加了 d-半乳糖诱导的老年和正常大鼠睾丸中 CYP19A1 和降低 AR 的表达。这些结果表明，雌激素介导的作用可能负责改善老年睾丸中的精子发生。此外，维生素 D3 可能在老年睾丸中具有保护作用，并且维生素 D3 处理的正常大鼠中不受影响的精子发生可能是由于雌激素和雄激素作用之间的平衡。
