PPAR-γ2 基因 Pro12Ala 多态性与伊朗 2 型糖尿病非酒精性脂肪性肝病患者的患病风险增加无关。
The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated with an increased risk of NAFLD in Iranian patients with type 2 diabetes mellitus.
机构信息
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Farhangian University, Shahid Mofatteh Teacher Education Paradise, Tehran, Iran.
出版信息
Cell Mol Biol Lett. 2019 Mar 15;24:12. doi: 10.1186/s11658-019-0138-0. eCollection 2019.
BACKGROUND
The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. Several studies have demonstrated a significant association between Pro12Ala polymorphism of the PPAR-γ2 gene and metabolic disorders. Therefore, this study aimed to evaluate the association of Pro12Ala polymorphism with increased risk of NAFLD in Iranian patients with type 2 diabetes mellitus.
METHODS
This cross-sectional study was performed on 145 healthy control subjects and 145 NAFLD patients with a history of type 2 diabetes. Pro12Ala polymorphism genotyping was performed using PCR-restriction fragment length polymorphism (RFLP) technique with the restriction enzyme.
RESULTS
Our results demonstrated that CC and GG genotypes of Pro12Ala were found in the participants, but there was no statistically significant difference between NAFLD patients and healthy controls ( = 0.64 and χ = 0.21).
CONCLUSION
This study suggests that Pro12Ala polymorphism of the PPAR-γ2 gene cannot be considered as a risk factor for NAFLD in the Iranian population.
背景
过氧化物酶体增殖物激活受体（PPARs）是配体激活的转录因子，属于核激素受体超家族。几项研究表明，PPAR-γ2 基因 Pro12Ala 多态性与代谢紊乱之间存在显著关联。因此，本研究旨在评估 Pro12Ala 多态性与伊朗 2 型糖尿病患者非酒精性脂肪性肝病（NAFLD）风险增加之间的关系。
方法
本横断面研究纳入了 145 名健康对照者和 145 名有 2 型糖尿病病史的 NAFLD 患者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，用限制性内切酶对 Pro12Ala 多态性进行基因分型。
结果
我们的结果表明，参与者中存在 Pro12Ala 的 CC 和 GG 基因型，但 NAFLD 患者与健康对照组之间无统计学显著差异（ = 0.64 和 χ = 0.21）。
结论
本研究提示，PPAR-γ2 基因 Pro12Ala 多态性不能被认为是伊朗人群中 NAFLD 的危险因素。
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