Department of Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Benha University, Benha City, Qalubia, Egypt.
Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Benha University, Benha City, Qalubia, Egypt.
J Biochem Mol Toxicol. 2019 Jul;33(7):e22321. doi: 10.1002/jbt.22321. Epub 2019 Mar 29.
miR-122 and miR-192 were investigated as indicators of toxic liver injury caused by acetaminophen, but their role in idiosyncratic toxic liver injury remains controversial. So, this work aimed to assess and compare the expressions of miR-122 and miR-192 in two different types of toxic liver injury (intrinsic [acetaminophen] and idiosyncratic [diclofenac]). Forty male adult Wistar albino rats were divided into equal five groups, in which serum liver enzymes; microRNAs (miRNAs) expressions (miR-122 and miR-192) and histopathological findings were studied. The present study showed that (1) miR-122 and miR-192 are good serum biomarkers of toxic liver injury whatever its etiology, as their serum levels exhibited a significantly earlier increase and earlier return to normal baseline levels as compared to serum aminotransferase levels; (2) miR-122 is more specific than miR-192; and (3) both serum levels of miR-122 and miR-192 showed non-significant differences in relation to the type of toxic liver injury.
miR-122 和 miR-192 被研究作为对乙酰氨基酚引起的肝毒性损伤的指标，但它们在特发性肝毒性损伤中的作用仍存在争议。因此，本研究旨在评估和比较两种不同类型的肝毒性损伤（内在型[乙酰氨基酚]和特发型[双氯芬酸]）中 miR-122 和 miR-192 的表达。将 40 只成年雄性 Wistar 白化大鼠等分为 5 组，研究血清肝酶；microRNAs（miRNAs）表达（miR-122 和 miR-192）和组织病理学发现。本研究表明：（1）miR-122 和 miR-192 是肝毒性损伤的良好血清生物标志物，无论其病因如何，其血清水平的升高和恢复正常基线水平的时间均早于血清转氨酶水平；（2）miR-122 比 miR-192 更具特异性；（3）miR-122 和 miR-192 的血清水平与肝毒性损伤的类型无关，差异无统计学意义。
