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利用气体传感器检测伤口中细菌释放的挥发性化合物。

Detection of Volatile Compounds Emitted by Bacteria in Wounds Using Gas Sensors.

机构信息

eVIDA Research Group, University of Deusto, 48007 Bilbao, Spain.

Department of Computer Engineering and Computer Science (CECS), University of Louisville, Louisville, KY 40292, USA.

出版信息

Sensors (Basel). 2019 Mar 28;19(7):1523. doi: 10.3390/s19071523.

DOI:10.3390/s19071523
PMID:30925832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6480681/
Abstract

In this paper we analyze an experiment for the use of low-cost gas sensors intended to detect bacteria in wounds using a non-intrusive technique. Seven different genera/species of microbes tend to be present in most wound infections. Detection of these bacteria usually requires sample and laboratory testing which is costly, inconvenient and time-consuming. The validation processes for these sensors with nineteen types of microbes (1 , 2 , 6 , 1 , 1 , 2 and 6 ) are presented here, in which four sensors were evaluated: TGS-826 used for ammonia and amines, MQ-3 used for alcohol detection, MQ-135 for CO₂ and MQ-138 for acetone detection. Validation was undertaken by studying the behavior of the sensors at different distances and gas concentrations. Preliminary results with liquid cultures of 10⁸ CFU/mL and solid cultures of 10⁸ CFU/cm of the 6 strains revealed that the four gas sensors showed a response at a height of 5 mm. The ammonia detection response of the TGS-826 to showed the highest responses for the experimental samples over the background signals, with a difference between the values ​​of up to 60 units in the solid samples and the most consistent and constant values. This could suggest that this sensor is a good detector of , and the recording made of its values ​​could be indicative of the detection of this species. All the species revealed similar CO₂ emission and a high response rate with acetone for , and .

摘要

在本文中,我们分析了一项使用低成本气体传感器检测伤口中细菌的实验,该实验采用非侵入性技术。大多数伤口感染中通常存在七种不同的属/种微生物。这些细菌的检测通常需要进行样本和实验室测试,这既昂贵、不便又耗时。这里呈现了这些传感器与 19 种微生物(1、2、6、1、1、2 和 6)的验证过程,其中评估了四种传感器:用于检测氨和胺的 TGS-826、用于检测酒精的 MQ-3、用于检测 CO₂的 MQ-135 和用于检测丙酮的 MQ-138。通过研究传感器在不同距离和气体浓度下的行为来进行验证。对 10⁸ CFU/mL 的液体培养物和 10⁸ CFU/cm 的 6 株固体培养物进行初步研究后发现,四种气体传感器在 5 毫米高度处均有响应。TGS-826 对 显示出的氨检测响应在实验样本中相对于背景信号具有最高的响应,在固体样本中,数值之间的差异高达 60 个单位,并且具有最一致和恒定的值。这可能表明该传感器是检测 的良好探测器,并且对其值的记录可能表明检测到了该物种。所有物种都表现出类似的 CO₂排放和对丙酮的高响应率,适用于 、 和 。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35d3/6480681/27a21de2c12b/sensors-19-01523-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35d3/6480681/27a21de2c12b/sensors-19-01523-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35d3/6480681/deac61d904d7/sensors-19-01523-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35d3/6480681/17aa2cd4e047/sensors-19-01523-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35d3/6480681/27a21de2c12b/sensors-19-01523-g010.jpg

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