Makhmudi Akhmad, Kalim Alvin Santoso
Pediatric Surgery Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Jl. Kesehatan No. 1, Yogyakarta, 55281, Indonesia.
BMC Res Notes. 2019 Mar 29;12(1):189. doi: 10.1186/s13104-019-4227-y.
Biliary atresia (BA) is the most common cause of neonatal jaundice, characterized by progressive and rapid liver fibrosis. Recent studies have shown that microRNAs (miRNAs) contribute to the liver fibrogenesis. We investigated the miRNA-21 impact in liver fibrogenesis in Indonesian BA patients.
There were 5, 4, and 7 BA patients with type 2A, 2B, and 3, respectively. Quantitative real-time polymerase chain reaction (qPCR) showed that the miRNA-21 expression was significantly increased (18-fold) in BA patients compared to controls (- 4.4 ± 4.0 vs. - 0.2 ± 4.8; p = 0.041). Furthermore, the phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression was significantly down-regulated (3.1-fold) in BA group compared to control group (0.2 ± 1.4 vs. - 1.4 ± 1.7; p = 0.036). The α-smooth muscle actin (α-SMA) expression was not statistically significantly different between groups (13.7 ± 3.8 vs. 15.0 ± 4.8; p = 0.87). Interestingly, the miRNA-21 expression was significantly lower (25-fold) in cirrhosis than non-cirrhosis BA patients (- 0.8 ± 2.2 vs. - 5.3 ± 3.9; p = 0.004). In conclusions, our study provides support for the association between miRNA-21 expression and liver cirrhosis in BA patients. Further study with a larger sample size of patients is important to confirm our results.
胆道闭锁（BA）是新生儿黄疸最常见的病因，其特征为进行性和快速的肝纤维化。最近的研究表明，微小RNA（miRNA）参与肝纤维化的发生。我们研究了miRNA-21对印度尼西亚BA患者肝纤维化的影响。
分别有5例、4例和7例BA患者，其类型为2A、2B和3型。定量实时聚合酶链反应（qPCR）显示，与对照组相比，BA患者中miRNA-21的表达显著增加（18倍）（-4.4±4.0对-0.2±4.8；p=0.041）。此外，与对照组相比，BA组中第10号染色体上缺失的磷酸酶和张力蛋白同源物（PTEN）的表达显著下调（3.1倍）（0.2±1.4对-1.4±1.7；p=0.036）。两组之间α平滑肌肌动蛋白（α-SMA）的表达无统计学显著差异（13.7±3.8对15.0±4.8；p=0.87）。有趣的是，肝硬化BA患者中miRNA-21的表达显著低于非肝硬化BA患者（25倍）（-0.8±2.2对-5.3±3.9；p=0.004）。总之，我们的研究为BA患者中miRNA-21表达与肝硬化之间的关联提供了支持。进一步对更大样本量的患者进行研究对于证实我们的结果很重要。
