Lazarus S C, McCabe L J, Nadel J A, Gold W M, Leikauf G D
Am J Physiol. 1986 Sep;251(3 Pt 1):C387-94. doi: 10.1152/ajpcell.1986.251.3.C387.
We examined the interaction between mast cell-derived mediators and the electrical and ion transport properties of canine tracheal epithelium. We compared the effect of mediators released by immunologic challenge of sensitized lung parenchyma with that of mediators released from canine mastocytoma cells challenged with calcium ionophore A23187. Short-circuit current (Isc) increased by 19.2 +/- 3.0 microA/cm2 in response to mediators released from sensitized lung fragments challenged with ragweed antigen. This effect was not due to histamine. When the epithelial tissues were pretreated with indomethacin, the same mediator supernatant increased Isc by only 3.8 +/- 4.3 microA/cm2. The mediators released from 10(7) mastocytoma cells challenged with calcium ionophore increased Isc by 25.1 +/- 13.6 microA/cm2. In the presence of indomethacin, the Isc increased by 2.0 +/- 0.4 microA/cm2. Mastocytoma-derived mediators produced an increase in net chloride secretion without a significant effect on net sodium absorption. This study provides direct evidence that mast cell-derived mediators can stimulate epithelial ion transport in canine trachea and suggests that the effect is indirect and dependent on intact cyclooxygenase pathways in the tracheal epithelium.
我们研究了肥大细胞衍生介质与犬气管上皮细胞电特性和离子转运特性之间的相互作用。我们比较了致敏肺实质免疫激发释放的介质与用钙离子载体A23187激发的犬肥大细胞瘤细胞释放的介质的作用。用豚草抗原激发致敏肺组织片段释放的介质可使短路电流（Isc）增加19.2±3.0微安/平方厘米。这种作用并非由组胺引起。当上皮组织用消炎痛预处理时，相同的介质上清液仅使Isc增加3.8±4.3微安/平方厘米。用钙离子载体激发10⁷个肥大细胞瘤细胞释放的介质使Isc增加25.1±13.6微安/平方厘米。在消炎痛存在的情况下，Isc增加2.0±0.4微安/平方厘米。肥大细胞瘤衍生的介质使净氯分泌增加，而对净钠吸收无显著影响。本研究提供了直接证据，表明肥大细胞衍生的介质可刺激犬气管上皮细胞的离子转运，并提示这种作用是间接的，且依赖于气管上皮中完整的环氧化酶途径。
