Leikauf G D, Ueki I F, Widdicombe J H, Nadel J A
Am J Physiol. 1986 Jan;250(1 Pt 2):F47-53. doi: 10.1152/ajprenal.1986.250.1.F47.
We examined whether the leukotrienes (LT) B4, C4, D4, and E4, and 5-, 12-, and 15-hydroxyeicosatetraenoic (HETE) acids alter the electrical properties of canine tracheal epithelium. Short-circuit current (ISC) increased by 20.3 +/- 4.3 and 25.9 +/- 6.5 microA/cm2 after LTC4 and LTD4 addition, was unchanged following LTB4, LTE4, 5-, or 15-HETE addition, and was decreased by 10 +/- 3% after 12-HETE addition. LTC4- and LTD4-induced increases in ISC were dependent on the presence of Cl and were equal to the increases in net 36Cl flux toward the mucosa. The cyclooxygenase inhibitor indomethacin (10(-5) M) or the phospholipase inhibitor mepacrine (10(-5) M) diminished increases in ISC. The release rate of prostaglandins E2 and F2 alpha and thromboxane B2 rose from 8.2 +/- 2.3, 2.3 +/- 0.5, and 1.1 +/- 0.5 to 15.5 +/- 3.3, 6.0 +/- 1.4, and 4.0 +/- 0.8 ng . cm-2 . h-1, respectively, after LTC4 addition, and from 6.9 +/- 1.4, 2.5 +/- 1.1, and 1.7 +/- 1.3 to 20.2 +/- 3.1, 10.5 +/- 3.1, and 5.2 +/- 2.4 ng . cm-2 . h-1, respectively, after LTD4 addition. The release rates of 6-keto-prostaglandin F1 alpha were unchanged. A thromboxane A2 mimetic, U46619, failed to increase ISC and thromboxane synthetase inhibitors OKY-046 and dazmegrel failed to inhibit the induced increases in ISC, suggesting that thromboxane has little effect on ISC. Thus lipoxygenase products of arachidonic acid can modulate Cl secretion by the canine tracheal epithelium and do so through the release of cyclooxygenase products, including prostaglandins E2 and F2 alpha.
我们研究了白三烯（LT）B4、C4、D4和E4，以及5-、12-和15-羟基二十碳四烯酸（HETE）是否会改变犬气管上皮的电特性。添加LTC4和LTD4后，短路电流（ISC）分别增加了20.3±4.3和25.9±6.5μA/cm2，添加LTB4、LTE4、5-或15-HETE后ISC不变，而添加12-HETE后ISC降低了10±3%。LTC4和LTD4诱导的ISC增加依赖于Cl的存在，且与向黏膜的净36Cl通量增加量相当。环氧化酶抑制剂吲哚美辛（10−5 M）或磷脂酶抑制剂米帕林（10−5 M）可减少ISC的增加。添加LTC4后，前列腺素E2、F2α和血栓素B2的释放速率分别从8.2±2.3、2.3±0.5和1.1±0.5 ng·cm−2·h−1升至15.5±3.3、6.0±1.
