Avantha Centre for Industrial Research and Development, Paper Mill Campus, Yamuna Nagar 135001, Haryana, India.
Int J Biol Macromol. 2019 Jul 1;132:166-177. doi: 10.1016/j.ijbiomac.2019.03.202. Epub 2019 Mar 27.
Bacterial nanocellulose (BNC), a natural origin biopolymer with multi-dimensional applications has captured a great deal of attention owing to its implausible properties. However, low yield of BNC accompanied with high production cost is challenging its usage in various technological applications. In this study, BNC production has been reported utilizing fermented black tea broth brewed from fresh tea leaves (FBTB) as well as from used tea leaves (FBTB) as a cost-effective and high-quality BNC yielding medium. The symbiotic combination of bacteria and yeasts (SCOBY) was exploited here to bring fermentation in tea broth. The production yields on dry weight basis were 13.3 g L in FBTB and 12.8 g L in FBTB, obtained with 60 g L of glucose in 20 days. The conversion yields of 0.32 and 0.31 g BNC/g sugar were obtained with both the tea broths. The study of produced pellicle using ATR-FTIR, FE-SEM and XRD confirmed its structural, morphological and chemical nature similar to that of BNC. Thus, fermented black tea broth appears to be a potential medium for BNC production. The use of fermented tea broth for the industrial scale production of BNC might significantly reduce its production cost.
细菌纳米纤维素（BNC）是一种具有多维应用的天然生物聚合物，由于其难以置信的特性而引起了广泛关注。然而，BNC 的低产量伴随着高生产成本，这对其在各种技术应用中的使用构成了挑战。在本研究中，利用新鲜茶叶（FBTB）和用过的茶叶（FBTB）发酵的红茶汤作为一种具有成本效益和高产 BNC 的优质培养基来报道 BNC 的生产。在这里，利用细菌和酵母的共生组合（SCOBY）来进行茶汁发酵。在 20 天内，在 60g/L 的葡萄糖条件下，FBTB 和 FBTB 中的产量分别达到了 13.3g/L 和 12.8g/L，以干重为基础。两种茶汁的 BNC 转化率分别为 0.32g 和 0.31g。使用 ATR-FTIR、FE-SEM 和 XRD 对生产的薄膜进行研究，证实其结构、形态和化学性质与 BNC 相似。因此，发酵红茶汤似乎是 BNC 生产的潜在培养基。在工业规模上使用发酵茶汁生产 BNC 可能会显著降低其生产成本。
