Sharma Chhavi, Bhardwaj Nishi K, Pathak Puneet, Dey Pinaki, Gautam Sneh, Kumar Samit, Dutt Purohit Shiv
University Institute of Biotechnology, Chandigarh University, Mohali 140413, India; University Centre for Research and Development, Chandigarh University, Mohali, -140413, India.
Avantha Centre for Industrial Research and Development, Yamuna Nagar-135001, Haryana, India.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134228. doi: 10.1016/j.ijbiomac.2024.134228. Epub 2024 Jul 27.
Bacterial nanocellulose was produced here using static, static intermittent-fed batch (SIFB) and rotary disc bioreactor (RDB) mode. Economical black tea broth media with symbiotic consortia of bacteria and yeast (SCOBY) was used towards feasible BNC production (instead of commercial NCIM 2526 strain and conventional HS media). The physicochemical characterization of BNC produced in all three modes via FE-SEM, ATR-FTIR, XRD and TGA results showed a highly porous morphology, mostly Iα form, good crystallinity and thermal stability, respectively. BNC crystallinity lies in the range of 68 % (RDB) to 79.4 % (static and SIFB). Water retention value (86 to 93 %) and moisture content (85 to 93 %) are high for BNC produced in all three modes. Commendable difference in the BNC yield, sugar consumption, conversion yield and residual sugar was observed using different methods. Highest BNC yield 29.4 ± 0.66 gL was obtained under SIFB method as compared to static mode (13.6 ± 0.32 g L). Under RDB, a negligible amount of BNC i.e., 1.0 ± 0.2 g L was produced. SCOBY with BTB medium was found unsuitable for BNC production under RDB and needs further investigation. Thus, this comparative study offers a way to produce a commendable amount of low-priced BNC for various techno-industrial usage.
本文采用静态、静态间歇补料分批培养（SIFB）和旋转圆盘生物反应器（RDB）模式生产细菌纳米纤维素。使用含有细菌和酵母共生菌群（SCOBY）的经济实惠的红茶肉汤培养基来实现可行的细菌纳米纤维素生产（而非商业NCIM 2526菌株和传统的HS培养基）。通过场发射扫描电子显微镜（FE-SEM）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）、X射线衍射（XRD）和热重分析（TGA）对三种模式下生产的细菌纳米纤维素进行的物理化学表征结果分别显示出高度多孔的形态、主要为Iα型、良好的结晶度和热稳定性。细菌纳米纤维素的结晶度在68%（RDB）至79.4%（静态和SIFB）范围内。三种模式下生产的细菌纳米纤维素的保水值（86%至93%）和水分含量（85%至93%）都很高。使用不同方法时，在细菌纳米纤维素产量、糖消耗、转化率和残余糖方面观察到了显著差异。与静态模式（13.6±0.32 g/L）相比，SIFB方法下获得了最高的细菌纳米纤维素产量29.4±0.66 g/L。在RDB模式下，产生的细菌纳米纤维素量可忽略不计，即1.0±0.2 g/L。发现带有红茶肉汤培养基的SCOBY在RDB模式下不适合用于细菌纳米纤维素生产，需要进一步研究。因此，这项比较研究提供了一种方法，可为各种技术工业用途生产出可观数量的低价细菌纳米纤维素。
