Laboratory of Neurosciences, Instituto Nacional de Pediatría (INP), Mexico City, Mexico.
Laboratory of Experimental Bacteriology, INP, Mexico City, Mexico.
Int J Biol Macromol. 2019 Jul 1;132:230-235. doi: 10.1016/j.ijbiomac.2019.03.200. Epub 2019 Mar 27.
The aim was to determine the effect of zinc (Zn) and insulin on oxidative stress and levels of dopamine in brain of rats. Wistar rats were treated either with zinc alone or combined with insulin during 10 days. After the last dose blood glucose was measured. Their brains were extracted to measure HO, Ca, Mg ATPase, glutathione (GSH), lipid peroxidation (Tbars) and Dopamine. Zn does not possess anti-glycemic effect like Insulin however, it is noticeable that the combination of Insulin plus Zn induces a major glucose reduction (p < 0.0001) than Insulin alone. In cerebellum/medulla oblongata, the groups treated with Insulin and Zn show a significantly increase in dopamine (p < 0.005). Insulin plus Zn reduced GSH level in cortex. Insulin plus Zn reduced level of HO in Striatum and in cerebellum/medulla oblongata. Lipid peroxidation was significantly reduced by the administration of Insulin as in the combination of Insulin and Zn in all regions (p < 0.0001). In cerebellum medulla oblongata, ATPase activity showed an increase only in the group treated with Insulin + Zn. CONCLUSION: These results suggest that the use of insulin plus Zn produce favorable changes on oxidative stress and this as consequence on the levels of dopamine.
目的是确定锌（Zn）和胰岛素对大鼠大脑氧化应激和多巴胺水平的影响。Wistar 大鼠在 10 天内单独用锌或与胰岛素联合治疗。最后一次给药后测量血糖。提取他们的大脑以测量 HO、Ca、Mg ATPase、谷胱甘肽 (GSH)、脂质过氧化 (Tbars) 和多巴胺。锌不像胰岛素那样具有抗血糖作用，然而，值得注意的是，胰岛素加锌的组合比单独使用胰岛素诱导出更大的葡萄糖减少（p < 0.0001）。在小脑/延髓，用胰岛素和 Zn 治疗的组显示多巴胺明显增加（p < 0.005）。胰岛素加 Zn 降低皮质 GSH 水平。胰岛素加 Zn 降低纹状体和小脑/延髓中的 HO 水平。脂质过氧化在所有区域均通过胰岛素的给药显著降低，如胰岛素和 Zn 的组合（p < 0.0001）。在小脑延髓，仅在胰岛素+Zn 治疗组中观察到 ATPase 活性增加。结论：这些结果表明，使用胰岛素加 Zn 可对氧化应激产生有利的变化，从而对多巴胺水平产生影响。
