School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, People's Republic of China.
School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jun 15;217:122-127. doi: 10.1016/j.saa.2019.03.081. Epub 2019 Mar 25.
A facile and rapid colorimetric approach was described for selective and sensitive determination of adenosine triphosphate (ATP) based on a hairpin aptamer probe and the anti-aggregation of AuNPs. Poly(diallyldimethylammonium chloride) (PDDA) can induce the aggregation of AuNPs due to the electrostatic interaction causing a red to blue color change. Upon the addition of ATP, aptamer-based hairpin probe is opened and releases flexible ssDNA ends. The released flexible ssDNA ends can interact with PDDA and prevent PDDA-induced AuNPs aggregation. Thus, a visible color change from blue to red and a decrease in the absorption ratio (A/A) are observed. Under the optimal conditions, the hairpin aptamer-based colorimetric assay exhibits high sensibility and selectivity for the detection of ATP with a detection limit of 1.7nM. Moreover, this assay is successfully used in the rapid determination of ATP in spiked human serum samples with good recoveries in the range of 102.88 to 104.07%.
一种基于发夹型适体探针和金纳米粒子（AuNPs）的抗聚集作用的简便、快速比色法，用于选择性和灵敏地测定三磷酸腺苷（ATP）。聚二烯丙基二甲基氯化铵（PDDA）由于静电相互作用会诱导 AuNPs 聚集，导致颜色从红色变为蓝色。加入 ATP 后，基于适体的发夹型探针被打开并释放出柔性 ssDNA 末端。释放的柔性 ssDNA 末端可以与 PDDA 相互作用，防止 PDDA 诱导的 AuNPs 聚集。因此，可以观察到从蓝色到红色的可见颜色变化和吸收比（A/A）的降低。在最佳条件下，基于发夹适体的比色法对 ATP 的检测具有高灵敏度和选择性，检测限低至 1.7nM。此外，该方法成功地用于测定加标人血清样品中的 ATP，回收率在 102.88%至 104.07%之间。
