The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, Shandong, China.
The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, 273165, Shandong, China.
Mikrochim Acta. 2018 Jul 5;185(8):359. doi: 10.1007/s00604-018-2888-1.
This study reports on a method for fluorometric aptasensing of adenosine triphosphate (ATP). It is based on the interaction of dispersed (red) and agglomerated (blue) gold nanoparticles (AuNPs) with a water-dispered terbium(III) based metal-organic framework (Tb-MOF). The dispersed AuNPs quench the emissions of the Tb-MOF, while the aggregated AuNPs have little effect. Under the condition of high salt concentration, the free aptamer against ATP does not stabilize the AuNPs against aggregation. This causes a color change from red to blue and weak quenching of the fluorescence of the Tb-MOF (with peaks at 489 nm and 544 nm after excitation at 290 nm). On addition of ATP, it will be bound by its aptamer to form a complex that is adsorbed on the AuNPs. This protects the AuNPs from salt-induced aggregation and the color (with a peak at 525 nm) remains red. The two fluorescence bands of the Tb-MOF are therefore suppressed by fluorescence resonance energy transfer (FRET) between Tb-MOF and the dispersed AuNPs. Fluorescence drops linearly in the 50 nM to 10 μM ATP concentration range, and the detection limit is 23 nM. ATP analogs such as guanosine triphosphate, uridine triphosphate, cytidine triphosphate, adenosine monophosphate and cyclic adenosine monophosphate have no obvious interference. The method was successfully applied to the determination of ATP in (spiked) human plasma samples and gave satisfactory recoveries. Graphical abstract Schematic of a terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. The dispersed gold nanoparticles (AuNPs) quench the fluorescence of the terbium-based metal-organic framework (Tb-MOF), while the aggregated AuNPs have little effect. In the presence of adenosine triphosphate (ATP), the aptamer-ATP complexes provide greater protection towards AuNPs than aptamer alone under high salt condition. Based on this, a novel Tb-MOF@AuNP platform is established for ATP detection.
本研究报告了一种基于分散（红色）和聚集（蓝色）金纳米粒子（AuNPs）与水分散的铽（III）基金属-有机骨架（Tb-MOF）相互作用的荧光适体传感检测三磷酸腺苷（ATP）的方法。分散的 AuNPs 猝灭 Tb-MOF 的发射，而聚集的 AuNPs 几乎没有影响。在高盐浓度条件下，游离的 ATP 适体不能稳定 AuNPs 使其不聚集。这导致颜色从红色变为蓝色，并且 Tb-MOF 的荧光猝灭减弱（在 290nm 激发下，峰值在 489nm 和 544nm 处）。加入 ATP 后，它将与其适体结合形成复合物，该复合物被吸附在 AuNPs 上。这保护 AuNPs 免受盐诱导的聚集，并且颜色（峰值在 525nm 处）保持红色。因此，Tb-MOF 的两个荧光带通过 Tb-MOF 和分散的 AuNPs 之间的荧光共振能量转移（FRET）被抑制。在 50nM 至 10μM ATP 浓度范围内，荧光呈线性下降，检测限为 23nM。ATP 类似物，如鸟嘌呤三磷酸、尿嘧啶三磷酸、胞嘧啶三磷酸、腺苷一磷酸和环腺苷一磷酸没有明显干扰。该方法成功应用于（加标）人血浆样品中 ATP 的测定，得到了令人满意的回收率。
