Center for Interstitial and Rare Lung Diseases, Department of Pneumology, Ruhrlandklinik University Hospital, University of Duisburg-Essen, Essen, Germany.
Tangshan Center for Disease Control and Prevention, Tangshan, China.
Respiration. 2019;98(1):29-37. doi: 10.1159/000496483. Epub 2019 Mar 29.
Anti-DFS70 antibodies, corresponding to the dense fine speckled antinuclear antibody (ANA) pattern in HEp-2 substrates, have been observed in chronic inflammatory conditions, cancer and in healthy individuals but in only a small percentage of patients with connective tissue diseases (CTD).
The study was aimed to investigate the possible role of Anti-DFS70 antibodies to distinguish CTD associated interstitial lung disease (CTD-ILD) from idiopathic interstitial pneumonia (IIP) and to explore potential correlations between anti-DFS70 antibodies and clinical parameters.
Serum samples were collected from 49 healthy controls (HC), 35 scleroderma-ILD (SSc-ILD) patients as negative controls for anti-DFS70 antibody, and 260 patients with the initial diagnosis IIP including 100 nonspecific interstitial pneumonia (NSIP) and 160 idiopathic pulmonary fibrosis (IPF) patients. ANA pattern was identified by indirect immunofluorescence on HEp-2 cells and anti-DFS70 antibodies were measured in serum by ELISA.
Serum anti-DFS70 antibodies were less frequently seen in ILD and SSc-ILD patients compared to HCs. Thirty-seven patients (34 initial idiopathic NSIP and 3 initial IPF patients) developed CTD during 24 months of follow-up, most of them combined with ANA positivity and anti-DFS70 antibody negativity. Anti-DFS70 antibody positivity was not significantly different between CTD-ILD and idiopathic ILD.
The frequency of serum anti-DFS70 antibody is markedly decreased in patients with ILDs. Anti-DFS70 antibodies may be useful to predict CTD development in ILD patients.
在慢性炎症性疾病、癌症和健康个体中观察到针对致密细斑点抗核抗体（ANA）模式的抗 DFS70 抗体，但在结缔组织疾病（CTD）患者中仅占一小部分。
本研究旨在探讨抗 DFS70 抗体在区分结缔组织疾病相关间质性肺病（CTD-ILD）与特发性间质性肺炎（IIP）中的可能作用，并探讨抗 DFS70 抗体与临床参数之间的潜在相关性。
收集了 49 名健康对照者（HC）、35 名硬皮病-ILD（SSc-ILD）患者（作为抗 DFS70 抗体的阴性对照）和 260 名初次诊断为 IIP 的患者血清样本，其中包括 100 名非特异性间质性肺炎（NSIP）和 160 名特发性肺纤维化（IPF）患者。通过在 HEp-2 细胞上进行间接免疫荧光鉴定 ANA 模式，并通过 ELISA 测量血清中的抗 DFS70 抗体。
ILD 和 SSc-ILD 患者血清中的抗 DFS70 抗体较 HC 组少见。在 24 个月的随访中，37 名患者（34 名初始特发性 NSIP 和 3 名初始 IPF 患者）发展为 CTD，其中大多数患者伴有 ANA 阳性和抗 DFS70 抗体阴性。CTD-ILD 和特发性 ILD 之间抗 DFS70 抗体阳性无显著差异。
ILD 患者血清中抗 DFS70 抗体的频率明显降低。抗 DFS70 抗体可能有助于预测 ILD 患者的 CTD 发展。
