von Ardenne M, Reitnauer P G
Onkologie. 1978 Aug;1(4):165-70. doi: 10.1159/000213942.
The time lapse of the effect of ifosfamid on the solid DS carcinosarcoma has been studied using 204 Wistar rats. The main results and the conclusions are as follows: 1. The transplantability of the tumor is abolished two hours after the i.v. application of 180 mg/kg isofamid (cessation of tumor cell proliferation). 2. Yet, the tumor tissue to be grafted is not damaged thoroughly by this treatment. It is possible that the still viable tumor cells were killed by the non-suppressed immune system of the recipients. 3. As determined by trypan blue dye exclusion and registration of glycolytic activity, the main part of tumor cells remains viable. As lately as 4 days after the therapy 80% of the cells incorporate trypan blue and the glycolytic activity is inhibited in the order of 80%. 4. It is to be expected that within two hours, a period sufficient for proliferation inhibition of tumor cells, only 30% of the active form of the drug administered can be found in the tumor. In this context the toxification (activation) kinetics of ifosfamid is discussed and an optimized, programmed infusion is considered. 5. The treatment with ifosfamid does not affect at least up to the third day the tumor hyperacidification attainable by a long-lasting glucose infusion.
使用204只Wistar大鼠研究了异环磷酰胺对实体DS癌肉瘤作用的时间间隔。主要结果和结论如下：1.静脉注射180mg/kg异环磷酰胺后两小时，肿瘤的移植性被消除（肿瘤细胞增殖停止）。2.然而，这种治疗并未彻底破坏待移植的肿瘤组织。有可能存活的肿瘤细胞被受体未受抑制的免疫系统杀死。3.通过台盼蓝染料排除法和糖酵解活性测定，肿瘤细胞的主要部分仍然存活。直到治疗后4天，80%的细胞摄取台盼蓝，糖酵解活性被抑制约80%。4.预计在两小时内，即足以抑制肿瘤细胞增殖的时间段内，在肿瘤中只能发现30%的给药活性形式的药物。在此背景下，讨论了异环磷酰胺的毒化（活化）动力学，并考虑了优化的程序化输注。5.至少在第三天之前，异环磷酰胺治疗不影响通过长期输注葡萄糖可实现的肿瘤过度酸化。
