Bowers Katherine, Markova Natalia
Principal Scientist/Group Leader Analytical and Formulation Development, FUJIFILM Diosynth Biotechnologies U.S.A., Inc., Morrisville, NC, USA.
MicroCal, Malvern Panalytical, Uppsala, Sweden.
Methods Mol Biol. 2019;1964:33-44. doi: 10.1007/978-1-4939-9179-2_3.
Differential scanning calorimetry (DSC) is a well-established technique for biomolecular stability studies. The technique is based on forced thermal denaturation of biomolecules in solution. Here we describe the use of DSC for characterization and optimization of stability of two proteins, a protein kinase and a mAb protein.
差示扫描量热法（DSC）是一种用于生物分子稳定性研究的成熟技术。该技术基于溶液中生物分子的强制热变性。在此，我们描述了使用DSC对两种蛋白质（一种蛋白激酶和一种单克隆抗体蛋白）的稳定性进行表征和优化。
