EFFECTS-262, Department of Medical Physiology, School of Medicine, University of Granada, Granada, Spain.
PROmoting FITness and Health through Physical Activity Research Group (PROFITH), Department of Physical Education and Sports, Faculty of Sport Sciences, University of Granada, Granada, Spain.
Scand J Med Sci Sports. 2019 Jul;29(7):910-921. doi: 10.1111/sms.13424. Epub 2019 Apr 21.
Maximal fat oxidation during exercise (MFO) and the exercise intensity eliciting MFO (Fat ) are considered biological markers of metabolic health and performance. A wide range of studies have been performed to increase our knowledge about their regulation by exercise and/or nutritional intervention. However, numerous data collection and analysis approaches have been applied, which may have affected the MFO and Fat estimation. We aimed to systematically review the available studies describing and/or comparing different data collection and analysis approach factors that could affect MFO and Fat estimation in healthy individuals and patients. Two independent researchers performed the search. We included all original studies in which MFO and/or Fat were estimated by indirect calorimetry through an incremental graded exercise protocol published from 2002 to 2019. This systematic review provides key information about the factors that could affect MFO and Fat estimation: ergometer type, metabolic cart used, warm-up duration and intensity, stage duration and intensities imposed in the graded exercise protocol, time interval selected for data analysis, stoichiometric equation selected to estimate fat oxidation, data analysis approach, time of the day when the test was performed, fasting time/previous meal before the test, and testing days for MFO/Fat and maximal oxygen uptake assessment. We suggest that researchers measuring MFO and Fat should take into account these key methodological issues that can considerably affect the accuracy, validity, and reliability of the measurement. Likewise, when comparing different studies, it is important to check whether the above-mentioned key methodological issues are similar in such studies to avoid ambiguous and unacceptable comparisons.
运动时最大脂肪氧化（MFO）和引发 MFO 的运动强度（Fat）被认为是代谢健康和表现的生物标志物。已经进行了广泛的研究来增加我们对运动和/或营养干预调节它们的认识。然而，已经应用了许多数据收集和分析方法，这可能会影响 MFO 和 Fat 的估计。我们旨在系统地回顾现有的研究，描述和/或比较可能影响健康个体和患者的 MFO 和 Fat 估计的不同数据收集和分析方法因素。两名独立研究人员进行了搜索。我们纳入了所有原始研究，这些研究通过递增分级运动方案通过间接热量法估计 MFO 和/或 Fat，这些研究发表于 2002 年至 2019 年。本系统综述提供了可能影响 MFO 和 Fat 估计的因素的关键信息：测力计类型、使用的代谢箱、热身持续时间和强度、递增运动方案中阶段的持续时间和强度、用于数据分析的时间间隔、用于估计脂肪氧化的化学计量方程、数据分析方法、测试进行的时间、测试前禁食时间/上次用餐、MFO/Fat 和最大摄氧量评估的测试天数。我们建议测量 MFO 和 Fat 的研究人员应考虑这些关键方法问题，这些问题会极大地影响测量的准确性、有效性和可靠性。同样，在比较不同的研究时，重要的是检查上述关键方法问题在这些研究中是否相似，以避免模糊和不可接受的比较。
