Department of Analytical Chemistry , National Institute of Chemistry , Hajdrihova 19 , SI-1000 Ljubljana , Slovenia.
Faculty of Chemistry and Chemical Technology , University of Ljubljana , Večna pot 113 , SI-1001 Ljubljana , Slovenia.
Anal Chem. 2019 May 7;91(9):6200-6205. doi: 10.1021/acs.analchem.9b00853. Epub 2019 Apr 10.
In the past decade, the development of single particle-inductively coupled plasma mass spectrometry (SP-ICPMS) has revolutionized the field of nanometallomics. Besides differentiation between dissolved and particulate metal signals, SP-ICPMS can quantify the nanoparticle (NP) number concentration and size. Because SP-ICPMS is limited to characterization of NPs in solution, we show how solid sampling by laser ablation (LA) adds spatial-resolution characteristics for localized NP analysis in biomaterials. Using custom-made gelatin standards doped with dissolved gold and commercial or synthesized gold nanoparticles, LA-SP-ICPMS conditions such as laser fluence, beam size, and dwell time were optimized for NP analysis to minimize NP degradation, peak overlap, and interferences from dissolved gold. A data-processing algorithm to retrieve the NP number concentration and size was developed for this purpose. As a proof-of-concept, a sunflower-root-sample cross-section, originating from a sunflower plant exposed to gold NPs, was successfully imaged using the optimized LA-SP-ICPMS conditions for localized NP characterization.
在过去的十年中，单颗粒电感耦合等离子体质谱（SP-ICPMS）的发展彻底改变了纳米金属组学领域。除了区分溶解态和颗粒态金属信号外，SP-ICPMS 还可以定量纳米颗粒（NP）的数浓度和粒径。由于 SP-ICPMS 仅限于对溶液中的 NPs 进行表征，我们展示了如何通过激光烧蚀（LA）进行固体采样，为生物材料中局部 NP 分析添加空间分辨率特征。使用含有溶解金的定制明胶标准品和商业或合成金纳米颗粒，优化了 LA-SP-ICPMS 的条件，如激光能量、光斑大小和停留时间，以最小化 NP 降解、峰重叠和溶解金的干扰，为此开发了一种数据处理算法来检索 NP 的数浓度和粒径。作为概念验证，成功地对暴露于金纳米颗粒的向日葵植株的向日葵根样本的横截面进行了局部 NP 特征的成像，使用了优化的 LA-SP-ICPMS 条件。
