巴西南部绵羊中巨型肉孢子虫大囊的出现及柔嫩肉孢子虫小囊的高频率感染
Occurrence of Sarcocystis gigantea macrocysts and high frequency of S. tenella microcysts in sheep from southern Brazil.
作者信息
Minuzzi Camila E, Cezar Alfredo S, Bräunig Patricia, Portella Luiza P, Rodrigues Fernando de S, Sangioni Luis Antonio, Vogel Fernanda S F
机构信息
Laboratório de Doenças Parasitárias (Ladopar), Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, prédio 44, sala 5139, 97105-900 Santa Maria, RS, Brazil.
Universidade Regional do Noroeste do Estado do Rio Grande do Sul (UNIJUÍ), Ijuí, RS, Brazil.
出版信息
Vet Parasitol Reg Stud Reports. 2019 Jan;15:100256. doi: 10.1016/j.vprsr.2018.12.002. Epub 2018 Dec 3.
The aim of this study was to investigate the occurrence of sarcocysts in sheep slaughtered in the Rio Grande do Sul state, Brazil. Heart and esophagus samples from 130 sheep were subjected to macroscopic and microscopic examination, followed by molecular analysis. Ten sheep (7.7%) had Sarcocystis gigantea macrocysts in esophagus, as identified by gene sequencing. Microcysts were present in 96.1% of the sheep, with a higher frequency (p < .05) in the heart (91.5%) compared to the esophagus (81.5%) samples. The microcysts were identified as Sarcocystis tenella by gene sequencing. Our results revealed a high frequency of Sarcocystis spp. infection in sheep from southern Brazil. To the authors knowledge, this is the first molecular confirmation of S. gigantea presence in Brazil.
本研究的目的是调查在巴西南里奥格兰德州屠宰的绵羊中肉孢子虫的发生情况。对130只绵羊的心脏和食管样本进行了宏观和微观检查，随后进行分子分析。通过基因测序鉴定，10只绵羊（7.7%）的食管中有巨型肉孢子虫大囊。96.1%的绵羊存在微囊，与食管样本（81.5%）相比，心脏样本中微囊的出现频率更高（p<0.05）（91.5%）。通过基因测序将微囊鉴定为柔嫩肉孢子虫。我们的结果显示巴西南部绵羊中肉孢子虫属感染的频率很高。据作者所知，这是巴西首次对巨型肉孢子虫的分子确认。
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