Department of Pharmacology, Therapeutics and Toxicology, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria.
Department of Pharmacology, Therapeutics and Toxicology, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria.
J Ethnopharmacol. 2019 Jun 28;238:111831. doi: 10.1016/j.jep.2019.111831. Epub 2019 Mar 28.
Trianthema portulacastrum L. (Aizoaceae) is used in traditional African Medicine for the treatment of various illnesses including dropsy, inflammation and rheumatism.
This study was designed to investigate the anti-nociceptive and anti-arthritic properties of the aqueous whole plant extract of Trianthema portulacastrum (AETP), possible mechanisms of action and characterize some of the active constituents.
Antinociceptive activity was evaluated using the acetic acid-induced writhing and hot plate tests in mice. The carrageenan test was used to induce a transient inflammation while arthritis was induced with complete Freund's adjuvant (CFA) in rats. On completion of CFA-induced arthritis macroscopic observations, the rats were euthanized to isolate the spleen, liver and limbs for estimation of oxidative stress and histological analysis.
AETP (10, 50, or 250 mg/kg; p.o.) produced significant (p < 0.05) and dose-dependent inhibition (41.10, 50.40, and 67.10%, respectively) of writhing response elicited by acetic acid. Also, increased pain threshold of supraspinally mediated nociceptive behaviour, with peak maximum possible effect (MPE) obtained at 250 mg/kg (22.98%; 30 min post-treatment). However, the pre-treatment of mice with Nitro-L-arginine (L-NNA) or naloxone reversed AETP-induced antinociception. In another experiment, AETP produced time course inhibition of carrageenan-induced paw oedema with peak effect (50.60%) at 250 mg/kg as well as significant reduction in CFA-induced arthritis by 58.56%, on day 27 and arthritic index (26.84%). Similarly, AETP attenuated CFA-induced MDA generation and deficit in antioxidant enzyme activities. Histological analysis of rat joints revealed a reduction in the synovial hyperplasia and mononuclear infiltration induced by CFA in AETP treated groups.
Findings from this study showed that T. portulacastrum possesses anti-nociceptive action through nitrergic and opioidergic signalling as well as anti-arthritic effect through enhancement of antioxidant defense system and inhibition of release or actions of inflammatory mediators.
蕺菜（马齿苋科）在传统的非洲医学中用于治疗各种疾病，包括水肿、炎症和风湿。
本研究旨在研究蕺菜全株水提物（AETP）的镇痛和抗关节炎特性、可能的作用机制，并对一些活性成分进行表征。
采用醋酸诱导扭体和热板试验评估镇痛活性。角叉菜胶试验用于诱导短暂性炎症，而弗氏完全佐剂（CFA）用于诱导大鼠关节炎。CFA 诱导关节炎完成后，对大鼠进行宏观观察，然后处死大鼠，分离脾脏、肝脏和四肢，用于评估氧化应激和组织学分析。
AETP（10、50 或 250mg/kg；po）对醋酸引起的扭体反应有显著（p<0.05）和剂量依赖性抑制作用，分别为 41.10%、50.40%和 67.10%。此外，AETP 还能增加脊髓介导的伤害性感受行为的疼痛阈值，最大可能效应（MPE）在 250mg/kg 时达到（22.98%；治疗后 30min）。然而，预先给予小鼠 L-精氨酸硝基（L-NNA）或纳洛酮可逆转 AETP 诱导的镇痛作用。在另一项实验中，AETP 对角叉菜胶诱导的足肿胀呈时间依赖性抑制，在 250mg/kg 时达到峰值（50.60%），并在第 27 天显著降低 CFA 诱导的关节炎（58.56%）和关节炎指数（26.84%）。同样，AETP 减轻了 CFA 诱导的 MDA 生成和抗氧化酶活性的降低。大鼠关节的组织学分析显示，AETP 治疗组可减少 CFA 诱导的滑膜增生和单核细胞浸润。
本研究结果表明，蕺菜具有镇痛作用，其作用机制涉及氮能和阿片能信号通路，还具有抗关节炎作用，其作用机制涉及增强抗氧化防御系统和抑制炎症介质的释放或作用。
