Levine Keith E, Collins Bradley J, Stout Matthew D, Wyde Michael, Afton Scott E, Essader Amal S, Ennis Todd J, Amato Kelly E, McWilliams Andrea C, Fletcher Brenda L, Fernando Reshan A, Harrington James M, Catlin Natasha, Robinson Veronica G, Waidyanatha Suramya
RTI International, 3040 E. Cornwallis Road, P.O. Box 12194, Research Triangle Park, NC, 27709, USA.
Division of the National Toxicology Program, National Institute of Environmental Health Sciences, MD EC-06, P.O. Box 12233, 111 T.W. Alexander Drive, Research Triangle Park, NC, 27709, USA.
Anal Lett. 2017;50(15):2447-2464. doi: 10.1080/00032719.2017.1293073. Epub 2017 Jun 22.
Zinc deficiency and excess can result in adverse health outcomes. There is conflicting evidence regarding whether excess or deficient zinc in the diet can contribute to carcinogenicity. The objective of this study was to characterize zinc carbonate basic for use as a source of dietary zinc in a rodent toxicity and carcinogenicity study investigating the effects of zinc deficiency and excess. Because of the complex chemistries of zinc carbonate basic compounds, inconsistent nomenclature, and literature and reference spectra gaps, it was necessary to employ multiple analytical techniques, including Karl Fischer titration, combustion analysis, inductively coupled plasma-optical emission spectrometry, X-ray diffraction, infrared spectroscopy, X-ray fluorescence spectrometry, and thermogravimetric analysis to characterize the test article. Based on the collective evidence and through the process of elimination, the test article was found to be composed mainly of zinc carbonate basic with zinc oxide as a minor component. The zinc content was determined to be 56.6% (w/w) with heavy metals such as arsenic, cadmium, mercury and lead below the limit of quantitation of less than or equal to 0.01%. The test material was stable at ambient temperature. Based on the work described in this manuscript, the test article was suitable for use as a source of zinc in studies of deficiency and excess in the diet.
锌缺乏和过量都会导致不良健康后果。关于饮食中锌过量或缺乏是否会导致致癌性，存在相互矛盾的证据。本研究的目的是在一项啮齿动物毒性和致癌性研究中，表征碱式碳酸锌作为膳食锌来源的特性，该研究旨在探究锌缺乏和过量的影响。由于碱式碳酸锌化合物的化学性质复杂、命名不一致以及文献和参考光谱存在空白，因此有必要采用多种分析技术，包括卡尔费休滴定法、燃烧分析法、电感耦合等离子体发射光谱法、X射线衍射法、红外光谱法、X射线荧光光谱法和热重分析法来表征受试物。基于综合证据并通过排除过程，发现受试物主要由碱式碳酸锌组成，氧化锌为次要成分。锌含量测定为56.6%（w/w），砷、镉、汞和铅等重金属含量低于或等于0.01%的定量限。受试材料在环境温度下稳定。基于本手稿中描述的工作，受试物适合用作饮食中锌缺乏和过量研究的锌源。
