Gül Amuk Nisa, Kurt Gökmen, Kartal Yandim Melis, Adan Aysun, Baran Yusuf
Department of Orthodontics, Faculty of Dentistry, Erciyes University , Kayseri , Turkey.
Department of Orthodontics, Faculty of Dentistry, Bezmiâlem Vakıf University , İstanbul , Turkey.
Turk J Biol. 2018 Oct 25;42(5):382-391. doi: 10.3906/biy-1712-62. eCollection 2018.
The aim of this study was to introduce a minimally invasive procedure for mesenchymal stem cell (MSC) transfer into the intact periodontal ligament (PDL) of the molar teeth in rats. Ten 12-week-old Wistar albino rats were used for this preliminary study. MSCs were obtained from bones of two animals and were labeled with green fluorescent protein (GFP). Four animals were randomly selected for MSC injection, while 4 animals served as a control group. Samples were prepared for histological analysis, Cox-2 mRNA expression polymerase chain reaction analysis, and fluorescent microscopy evaluation. The number of total cells, number of osteoclastic cells, and Cox-2 mRNA expression levels of the periodontal tissue of teeth were calculated. The number of total cells was increased with MSC injections in PDL significantly (P < 0.001). The number of osteoclastic cells and Cox-2 mRNA expression were found to be similar for the two groups. GFP-labeled MSCs were observed with an expected luminescence on the smear samples of the PDL with transferred MSCs. The results of this preliminary study demonstrate successful evidence of transferring MSCs to intact PDL in a nonsurgical way and offer a minimally invasive procedure for transfer of MSCs to periodontal tissues.
本研究的目的是引入一种将间充质干细胞（MSC）转移至大鼠磨牙完整牙周膜（PDL）的微创方法。本初步研究使用了10只12周龄的Wistar白化大鼠。从两只动物的骨骼中获取间充质干细胞并用绿色荧光蛋白（GFP）进行标记。随机选择4只动物进行间充质干细胞注射，另外4只动物作为对照组。制备样本用于组织学分析、Cox-2 mRNA表达聚合酶链反应分析以及荧光显微镜评估。计算牙齿牙周组织的总细胞数、破骨细胞数以及Cox-2 mRNA表达水平。在牙周膜中注射间充质干细胞后，总细胞数显著增加（P < 0.001）。发现两组的破骨细胞数和Cox-2 mRNA表达相似。在注射了间充质干细胞的牙周膜涂片样本上观察到了带有预期荧光的绿色荧光蛋白标记的间充质干细胞。本初步研究结果证明了以非手术方式成功将间充质干细胞转移至完整牙周膜，并为将间充质干细胞转移至牙周组织提供了一种微创方法。
