Amuk Nisa Gul, Kurt Gökmen, Baran Yusuf, Seyrantepe Volkan, Yandim Melis Kartal, Adan Aysun, Demir Secil Akyildiz, Kiraz Yagmur, Sonmez Mehmet Fatih
Department of Orthodontics, Faculty of Dentistry, Erciyes University, Kayseri.
Department of Orthodontics, Faculty of Dentistry, Istanbul Yeni Yüzyil University.
Eur J Orthod. 2017 Jun 1;39(3):235-242. doi: 10.1093/ejo/cjw054.
The aim of this study is to evaluate and compare therapeutic effects of mesenchymal stem cell (MSCs) and osteoprotegerin (OPG) gene transfer applications on inhibition and/or repair of orthodontically induced inflammatory root resorption (OIIRR).
Thirty Wistar rats were divided into four groups as untreated group (negative control), treated with orthodontic appliance group (positive control), MSCs injection group, and OPG transfected MSCs [gene therapy (GT) group]. About 100g of orthodontic force was applied to upper first molar teeth of rats for 14 days. MSCs and transfected MSC injections were performed at 1st, 6th, and 11th days to the MSC and GT group rats. At the end of experiment, upper first molar teeth were prepared for genetical, scanning electron microscopy (SEM), fluorescent microscopy, and haematoxylin eosin-tartrate resistant acid phosphatase staining histological analyses. Number of total cells, number of osteoclastic cells, number of resorption lacunae, resorption area ratio, SEM resorption ratio, OPG, RANKL, Cox-2 gene expression levels at the periodontal ligament (PDL) were calculated. Paired t-test, Kruskal-Wallis, and chi-square tests were performed.
Transferred MSCs showed marked fluorescence in PDL. The results revealed that number of osteoclastic cells, resorption lacunae, resorption area ratio, RANKL, and Cox-2 were reduced after single MSC injections significantly (P < 0.05). GT group showed the lowest number of osteoclastic cells (P < 0.01), number of resorption lacunae, resorption area ratio, and highest OPG expression (P < 0.001).
Taken together all these results, MSCs and GT showed marked inhibition and/or repair effects on OIIRR during orthodontic treatment on rats.
本研究旨在评估和比较间充质干细胞（MSCs）和骨保护素（OPG）基因转移应用对正畸诱导的炎性牙根吸收（OIIRR）的抑制和/或修复作用。
将30只Wistar大鼠分为四组，即未处理组（阴性对照）、正畸矫治器处理组（阳性对照）、MSCs注射组和OPG转染的MSCs组[基因治疗（GT）组]。对大鼠的上颌第一磨牙施加约100g的正畸力，持续14天。在第1天、第6天和第11天对MSCs组和GT组大鼠进行MSCs和转染的MSCs注射。实验结束时，对上颌第一磨牙进行基因、扫描电子显微镜（SEM）、荧光显微镜以及苏木精-伊红-抗酒石酸酸性磷酸酶染色组织学分析。计算牙周膜（PDL）中的总细胞数、破骨细胞数、吸收陷窝数、吸收面积比、SEM吸收比、OPG、RANKL、Cox-2基因表达水平。进行配对t检验、Kruskal-Wallis检验和卡方检验。
转染的MSCs在PDL中显示出明显的荧光。结果显示，单次注射MSCs后，破骨细胞数、吸收陷窝数、吸收面积比、RANKL和Cox-2均显著减少（P<0.05）。GT组的破骨细胞数最低（P<0.01），吸收陷窝数、吸收面积比最低，OPG表达最高（P<0.001）。
综合所有这些结果，MSCs和基因治疗在大鼠正畸治疗期间对OIIRR显示出明显的抑制和/或修复作用。
