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通过使用双花扁豆凝集素从荷瘤小鼠的脾细胞中富集在体内有效的抗肿瘤效应细胞。

Enrichment of antitumor effector cells that are effective in vivo from spleen cells of tumor-bearing mice through the use of Dolichos biflorus lectin.

作者信息

Okada T, Ezawa K, Imai Y, Osawa T

出版信息

Cancer Res. 1986 Nov;46(11):5611-7.

PMID:3093067
Abstract

Spleen cells of X5563 tumor-bearing mice were fractionated with Dolichos biflorus lectin (DBA) into DBA+ (agglutinable with DBA) and DBA- (nonagglutinable with DBA) fractions. The DBA- cells showed antitumor activity specific for X5563 cells in vivo when injected into mice together with X5563 cells (Winn assay) or injected i.p. into mice previously inoculated s.c. with X5563 cells (adoptive transfer) with simultaneous administration of interleukin-2. Both DBA+ and DBA- cells have the cell surface phenotype of Lyt-1-2+, but only DBA- cells exhibit antitumor activity in vivo, while DBA+ cells have stronger cytolytic activity against X5563 cells in vitro than DBA- cells. This cell separation method involving the use of DBA is simple and gives reproducible results and high yields of viable cells.

摘要

用双花扁豆凝集素（DBA）对荷X5563肿瘤小鼠的脾细胞进行分离，得到DBA +（可被DBA凝集）和DBA -（不可被DBA凝集）组分。当与X5563细胞一起注射到小鼠体内（温氏试验）或经皮下预先接种X5563细胞的小鼠腹腔注射（过继转移）并同时给予白细胞介素-2时，DBA -细胞在体内表现出对X5563细胞的抗肿瘤活性。DBA +和DBA -细胞均具有Lyt-1 - 2 +的细胞表面表型，但只有DBA -细胞在体内表现出抗肿瘤活性，而DBA +细胞在体外对X5563细胞的细胞溶解活性比DBA -细胞更强。这种涉及使用DBA的细胞分离方法简单，结果可重复，并且活细胞产量高。

相似文献

1
Enrichment of antitumor effector cells that are effective in vivo from spleen cells of tumor-bearing mice through the use of Dolichos biflorus lectin.通过使用双花扁豆凝集素从荷瘤小鼠的脾细胞中富集在体内有效的抗肿瘤效应细胞。
Cancer Res. 1986 Nov;46(11):5611-7.
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引用本文的文献

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Br J Cancer. 1990 Feb;61(2):241-9. doi: 10.1038/bjc.1990.45.
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Carbohydrate binding specificities of several poly-N-acetyllactosamine-binding lectins.
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Glycoconj J. 1990;7(4):323-34. doi: 10.1007/BF01073376.
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Surface glycoprotein of human natural killer cells recognized by wheat germ agglutinin.可被麦胚凝集素识别的人类自然杀伤细胞表面糖蛋白。
Glycoconj J. 1992 Aug;9(4):198-203. doi: 10.1007/BF00731165.