Fuyama S, Yamamoto H, Arai S
Cancer Res. 1985 Sep;45(9):4103-8.
Antitumor activity of spleen cells from BALB/c mice bearing RL male 1 lymphoma was studied. In the Winn assay spleen cells of the tumor-bearing mice inhibited the growth of RL male 1 lymphoma. This antitumor activity of spleen cells was not detected by the in vitro cell-mediated cytotoxicity assay (4-h 51Cr release assay). The effector cells in spleen cells were T-cells which manifested asialo GM1 on their cell surfaces and were radiosensitive (1000 rads). The analysis of T-cell subsets using Lyt markers showed that the effector cells expressed the Lyt-2 antigen with a small amount of the Lyt-1 antigen on their cell surfaces. In addition antitumor activity of spleen cells of the tumor-bearing mice was weak in the early stage of tumor growth, strong in the mid-stage, and disappeared in the late stage. The mechanisms of antitumor activity of spleen cells of the tumor-bearing mice are discussed.
对携带RL雄性1淋巴瘤的BALB/c小鼠脾细胞的抗肿瘤活性进行了研究。在Winn试验中，荷瘤小鼠的脾细胞抑制了RL雄性1淋巴瘤的生长。通过体外细胞介导的细胞毒性试验（4小时51Cr释放试验）未检测到脾细胞的这种抗肿瘤活性。脾细胞中的效应细胞是T细胞，其细胞表面表现为无唾液酸GM1，且对辐射敏感（1000拉德）。使用Lyt标记对T细胞亚群进行分析表明，效应细胞在其细胞表面表达Lyt-2抗原，同时伴有少量Lyt-1抗原。此外，荷瘤小鼠脾细胞的抗肿瘤活性在肿瘤生长早期较弱，中期较强，晚期消失。本文讨论了荷瘤小鼠脾细胞抗肿瘤活性的机制。
