Rifai N, Silverman L M
Clin Chem. 1986 Oct;32(10):1969-72.
Immunoturbidimetric assays for determination of serum apolipoproteins CII and CIII (apo CII and CIII) were developed for use on the Cobas-Bio centrifugal analyzer with commercially available antisera and reference sera. Within-run and between-run imprecision (CVs) for apo CII was respectively 3.3 and 6.2%, and for apo CIII 2.75 and 5.2%. Addition to sera of as much as 50 g of hemoglobin or 0.15 g of bilirubin per liter did not interfere with determinations of apolipoproteins. Concentrations of apo CII and CIII were measured in sera from 40 normolipidemic and hyperlipidemic subjects by these immunoturbidimetric methods (x) and by radial immunodiffusion assays (y). Apo CII and CIII gave slopes of 0.93 and 0.86, y-intercepts of 7.4 and 9.5 mg/L, and correlation coefficients of 0.96 and 0.94, respectively. Reference intervals for the two apolipoproteins were established with sera from 100 normolipidemic subjects. The distribution of apo CII and CIII among the various lipoprotein fractions for normal subjects differed significantly (p less than 0.005) from that for hypertriglyceridemic subjects.
采用市售抗血清和参考血清，开发了用于在Cobas-Bio离心分析仪上测定血清载脂蛋白CII和CIII（apo CII和CIII）的免疫比浊法。apo CII的批内和批间不精密度（CV）分别为3.3%和6.2%，apo CIII的批内和批间不精密度分别为2.75%和5.2%。每升血清中加入多达50 g血红蛋白或0.15 g胆红素不会干扰载脂蛋白的测定。通过这些免疫比浊法（x）和放射免疫扩散法（y）测量了40名血脂正常和血脂异常受试者血清中apo CII和CIII的浓度。apo CII和CIII的斜率分别为0.93和0.86，截距分别为7.4和9.5 mg/L，相关系数分别为0.96和0.94。用100名血脂正常受试者的血清建立了这两种载脂蛋白的参考区间。正常受试者和高甘油三酯血症受试者各种脂蛋白组分中apo CII和CIII的分布差异显著（p小于0.005）。
