Rifai N, Silverman L M
Clin Chim Acta. 1987 Mar 16;163(2):207-13. doi: 10.1016/0009-8981(87)90024-6.
Immunoturbidimetric assay for the determination of serum apolipoprotein E was developed on the Cobas-BIO centrifugal analyzer using commercially available antisera and reference sera. Within-run imprecision was 2.9% while day-to-day imprecision was 7.9%. Addition of up to 50 g/l hemoglobin or 0.15 g/l bilirubin to sera did not interfere with the determination of this apolipoprotein. Apolipoprotein E concentration was measured in sera from 40 normolipidemic and hyperlipidemic subjects by this immunoturbidimetric method (dependent variable) and radial immunodiffusion assay (independent variable). A slope of 0.90, an intercept of -7.9, and a correlation coefficient of 0.94 were obtained. Reference range for apolipoprotein E was established using sera from 100 normolipidemic subjects. The mean + 1 SD for apolipoprotein E levels of males and females were 36 + 13 mg/l and 29 + 8.4 mg/l, respectively. The distribution of apolipoprotein E among the various lipoprotein fractions of normals was significantly different than that of hypertriglyceridemic subjects.
利用市售抗血清和参考血清，在Cobas - BIO离心分析仪上开发了用于测定血清载脂蛋白E的免疫比浊法。批内不精密度为2.9%，而日间不精密度为7.9%。向血清中添加高达50 g/l的血红蛋白或0.15 g/l的胆红素不会干扰这种载脂蛋白的测定。通过这种免疫比浊法（因变量）和放射免疫扩散法（自变量）测定了40名血脂正常和高脂血症患者血清中的载脂蛋白E浓度。得到的斜率为0.90，截距为 -7.9，相关系数为0.94。使用100名血脂正常受试者的血清建立了载脂蛋白E的参考范围。男性和女性载脂蛋白E水平的均值 + 1标准差分别为36 + 13 mg/l和29 + 8.4 mg/l。正常人群各种脂蛋白组分中载脂蛋白E的分布与高甘油三酯血症患者的分布显著不同。
