豚鼠（豚鼠属）肝脏和表皮组氨酸酶的比较研究。
A comparative study of hepatic and epidermal histidase in the guinea-pig (Cavia porcellus).
作者信息
Allen R L, Hopewell R, Prottey C
出版信息
Comp Biochem Physiol B. 1986;84(4):523-9. doi: 10.1016/0305-0491(86)90117-3.
PMID:3093144
Abstract
Histidine ammonia lyase was purified to homogeneity from guinea-pig liver and epidermis. Both enzymes had similar molecular weights, subunit composition and pH optima. Km values for the two were similar at pH 9.2 but different at pH 7.0. Both enzymes were stimulated by low thiol concentrations and inhibited at higher concentrations, but to different extents. Antibody to the hepatic enzyme showed complete identity against hepatic enzyme but incomplete identity against epidermal enzyme.
摘要
从豚鼠肝脏和表皮中纯化出了均一的组氨酸解氨酶。两种酶具有相似的分子量、亚基组成和最适pH值。在pH 9.2时，二者的米氏常数相似，但在pH 7.0时不同。两种酶在低硫醇浓度下均受到刺激，而在高浓度下均受到抑制，但程度不同。针对肝脏酶的抗体与肝脏酶完全一致，但与表皮酶不完全一致。
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