Kuroda Y, Ito M, Ogawa T, Takeda E, Toshima K, Miyao M
Clin Chim Acta. 1979 Aug 15;96(1-2):139-44. doi: 10.1016/0009-8981(79)90063-9.
A new sensitive method was developed for assay of histidase in the stratum corneum of human skin with [14C] histidine as substrate. With this method histidase could be detected using less than 1 mg of stratum corneum. Skin histidase was markedly reduced in seven infants with histidinemia. The enzyme activities in thirteen parents of the patients were about 50% of the normal values, and even the highest values in the parents did not overlap the lowest normal values. Thus heterozygotes in the autosomal recessive form of histidinemia can be distinguished from normal persons by their skin histidase activity measured by this sensitive assay method.
以[14C]组氨酸为底物，开发了一种用于检测人皮肤角质层中组氨酸酶的新的灵敏方法。使用这种方法，用少于1毫克的角质层就能检测到组氨酸酶。7例组氨酸血症婴儿的皮肤组氨酸酶明显降低。13例患者父母的酶活性约为正常值的50%，甚至父母中的最高值也未与最低正常值重叠。因此，通过这种灵敏的检测方法测量皮肤组氨酸酶活性，可以将常染色体隐性形式组氨酸血症的杂合子与正常人区分开来。
