Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität München, 82152 Planegg, Martinsried, Germany.
Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Chemical Biology Department, Robert-Rössle-Strasse 10, 13125 Berlin, Germany and Humboldt Universität zu Berlin, Department of Chemistry, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
Org Biomol Chem. 2019 May 28;17(20):4964-4969. doi: 10.1039/c9ob00508k. Epub 2019 Apr 1.
We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody-fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.
我们介绍了一种化学酶促策略，用于直接在体外生成 C 端连接的融合蛋白。微管蛋白酪氨酸连接酶用于掺入互补的点击化学接头，从而促进功能性双特异性抗体片段的形成。这种简单的策略可以作为模块化蛋白连接平台的核心连接中心。
