Takaoka H
Hokkaido Igaku Zasshi. 1986 Jul;61(4):556-67.
Immature follicles grow autonomously even without pituitary gonadotropins. In order to explore this reason, promoting effects of oocyte and some endocrinological substances on the proliferation of granulosa-cells (G-cells) have been investigated using the labelling index (LI) with 3H-Thymidine (3H-Tdr) and mitotic index (MI) in mature mice and immature hypophysectomized (hypox) rats. The following results were obtained: In preantral follicles with the oocyte diameter below 69 mu, the LI was low (9.5 +/- 1.7%) (m +/- SE) and the follicular diameter was small (69.0 +/- 7.7 mu). As the oocyte developed with its diameter 80-89 mu, the LI increased (20.3 +/- 3.8%) and the follicular diameter also increased (192.3 +/- 19.4 mu). In preantral follicles with the oocyte diameter over 80 mu the G-cells near the oocyte had a much higher LI than the distant G-cells. After the antrum formation, the LI and MI of cumulus layers adjacent to the oocyte were three times higher than those of mural layers. Among the mural cell layers in the antral follicle, the layer adjacent to the antrum showed remarkably high LI in comparison with the distant layers. In FSH primed group the LI of G-cells was two fold higher than that in hypox control group (p less than 0.001). In the preantral follicle of hypox control group the G-cells near the oocyte had a much higher LI than the distant G-cells. Moreover in FSH primed group the LI indicated high value not only in the cell layers adjacent to the oocyte but also in the cell layers adjacent to the theca interna. In LH, E2 and P4 primed groups the LI of G-cells had no change significantly in comparison with that in hypox control group. In FSH + LH primed group the LI of G-cells decreased significantly in comparison with that in FSH primed group (p less than 0.05). In FSH + E2 group the LI had no change significantly in comparison with that in FSH primed group. These results suggest that the granulosa-cell growth factor (GGF) in oocyte, which seems to play a role in the formation of cumulus, is secreted with the growth of the oocyte. In antral follicles the GGF is transported through the follicular fluid.(ABSTRACT TRUNCATED AT 400 WORDS)
未成熟卵泡即使在没有垂体促性腺激素的情况下也能自主生长。为了探究其原因，利用³H-胸腺嘧啶核苷（³H-Tdr）标记指数（LI）和有丝分裂指数（MI），在成熟小鼠和未成熟垂体切除（hypox）大鼠中研究了卵母细胞和一些内分泌物质对颗粒细胞（G细胞）增殖的促进作用。得到了以下结果：在卵母细胞直径小于69μm的窦前卵泡中，LI较低（9.5±1.7%）（平均值±标准误），卵泡直径较小（69.0±7.7μm）。随着卵母细胞发育至直径80 - 89μm，LI升高（20.3±3.8%），卵泡直径也增大（192.3±19.4μm）。在卵母细胞直径超过80μm的窦前卵泡中，靠近卵母细胞的G细胞的LI远高于远处的G细胞。在卵泡腔形成后，与卵母细胞相邻的卵丘层的LI和MI比壁层高三倍。在卵泡腔卵泡的壁细胞层中，与卵泡腔相邻的层与远处的层相比显示出显著较高的LI。在FSH预处理组中，G细胞的LI比hypox对照组高两倍（p＜0.001）。在hypox对照组的窦前卵泡中，靠近卵母细胞的G细胞的LI远高于远处的G细胞。此外，在FSH预处理组中，LI不仅在与卵母细胞相邻的细胞层中显示出高值，而且在与内膜相邻的细胞层中也显示出高值。在LH、E2和P4预处理组中，G细胞的LI与hypox对照组相比无显著变化。在FSH + LH预处理组中，G细胞的LI与FSH预处理组相比显著降低（p＜0.05）。在FSH + E2组中，LI与FSH预处理组相比无显著变化。这些结果表明，卵母细胞中的颗粒细胞生长因子（GGF）似乎在卵丘形成中起作用，它随着卵母细胞的生长而分泌。在卵泡腔卵泡中，GGF通过卵泡液运输。（摘要截取自400字）
