Takaoka H, Satoh H, Makinoda S, Moriya S, Ichinoe K
Nihon Sanka Fujinka Gakkai Zasshi. 1985 Jan;37(1):92-8.
The possible existence of granulosa-cell growth factor (GGF) in the oocyte has been investigated using the labelling index (LI) with 3H-Thymidine and the mitotic index (MI) of granulosa cells (G-cells) in mice. The following results were obtained. In preantral follicles the LI increased with the development of the oocyte until its diameter reached the maximum (approximately 90 mu). In preantral follicles with the oocyte diameter below 69 mu, the LI was low and the follicular diameter was small. As the oocyte developed, the LI increased and reached the maximum value when the follicular diameter was approximately 200 mu. Then it decreased a little but maintained a high value. The antrum formation was seen when the follicular diameter was about 300 mu showing no change in LI. In preantral follicles with the oocyte diameter over 80 mu the G-cell near the oocyte had a much higher LI than the distant one. After the antrum formation, the LI and MI of cumulus layers adjacent to the oocyte were three times as great as those of the mural layers. Among the mural cell layers in the antral follicle the layer adjacent to the antrum showed remarkably high LI in comparison with the distant layers. These results suggest that GGF which seems to play a role in the formation of cumulus is secreted with the development of the oocyte. The GGF is transported through two systems the preantral follicle and cumulus layer through the G-cell gap junction and mural layers through the follicular fluid.
利用3H-胸腺嘧啶核苷标记指数（LI）和小鼠颗粒细胞（G细胞）的有丝分裂指数（MI），研究了卵母细胞中颗粒细胞生长因子（GGF）可能的存在情况。得到了以下结果。在窦前卵泡中，LI随着卵母细胞的发育而增加，直至其直径达到最大值（约90μm）。在卵母细胞直径小于69μm的窦前卵泡中，LI较低且卵泡直径较小。随着卵母细胞的发育，LI增加，当卵泡直径约为200μm时达到最大值。然后略有下降但仍保持较高值。当卵泡直径约为300μm时可见腔形成，此时LI无变化。在卵母细胞直径超过80μm的窦前卵泡中，靠近卵母细胞的G细胞的LI远高于远处的G细胞。腔形成后，与卵母细胞相邻的卵丘层的LI和MI是壁层的三倍。在窦状卵泡的壁细胞层中，与腔相邻的层与远处的层相比显示出明显较高的LI。这些结果表明，似乎在卵丘形成中起作用的GGF随着卵母细胞的发育而分泌。GGF通过两种系统运输，通过G细胞间隙连接在窦前卵泡和卵丘层中运输，通过卵泡液在壁层中运输。
