Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand; Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Carbohydr Res. 2019 May 15;477:11-19. doi: 10.1016/j.carres.2019.03.010. Epub 2019 Mar 23.
Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement. The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.
槐豆胶的酶解提供了一个 Manβ(1 → 4)Man 二糖，该二糖可以通过转化为 2-碘糖基叠氮化物和 Lafont 重排转化为所有 N-聚糖核心 Manβ(1 → 4)GlcNAc 二糖单元。Manβ(1 → 4)GlcNAc 二糖可作为关键中间体用于更复杂 N-聚糖结构的修饰，提供了一种 N-聚糖恶唑啉作为 ENGase 酶供体底物的途径，比以前报道的途径短得多。
