Research Center of Gansu Military and Civilian Integration Advanced Structural Materials, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education of China, Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
Research Center of Gansu Military and Civilian Integration Advanced Structural Materials, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education of China, Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
Bioelectrochemistry. 2019 Aug;128:74-82. doi: 10.1016/j.bioelechem.2019.03.006. Epub 2019 Mar 26.
Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading. Then, a novel composite was synthesized via β-cyclodextrin (β-CD) functionalized GO-Fc by combining the advantages of GO-Fc and β-CD. An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers. The successful synthesis of the composites was confirmed by FTIR, XRD, TGA, SEM, and XPS results. The host-guest inclusion interaction was detected by ultraviolet spectroscopy and DPV. The recognition results demonstrated that the rGO-Fc-CD/GCE showed a higher chiral recognition capability for L-Phe than for D-Phe. The enantioselectivity coefficient (I/I) of the proposed sensor was 2.47. The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine were obtained for this electrochemical sensor. The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
氧化石墨烯（GO）经二茂铁（Fc）修饰后通过π-π 相互作用（GO-Fc）成功组装，具有大表面积和高负载的特点。然后，通过结合 GO-Fc 和β-环糊精（β-CD）的优势，将β-CD 功能化的 GO-Fc 合成了一种新型复合材料。通过将 rGO-Fc-CD 复合材料用作电极修饰来构建高效的手性电化学传感界面，用于识别苯丙氨酸（Phe）对映体。FTIR、XRD、TGA、SEM 和 XPS 结果证实了复合材料的成功合成。通过紫外光谱和 DPV 检测到主体-客体包合相互作用。识别结果表明，rGO-Fc-CD/GCE 对 L-Phe 的手性识别能力高于 D-Phe。所提出的传感器的对映选择性系数（I/I）为 2.47。该电化学传感器对 L-苯丙氨酸和 D-苯丙氨酸的检测限分别为 27 nM 和 52 nM（S/N=3）。该合成材料成功地用于识别 Phe 对映体，表明所开发的传感器具有广泛的应用前景。
