Plant Stress Laboratory 204/207, Department of Molecular and Cell Biology, MCB Building, Upper Campus, University of Cape Town, Private bag X3, Rondebosch, Cape Town 7701, South Africa.
Department of Botany, School of Biological Sciences, University of Nairobi, P.O. Box 30197, Nairobi 00100, Kenya.
Toxins (Basel). 2019 Mar 25;11(3):179. doi: 10.3390/toxins11030179.
colonisation of maize can produce mycotoxins that are detrimental to both human and animal health. Screening of maize lines, resistant to infection, together with a biocontrol strategy, could help minimize subsequent aflatoxin contamination. We developed a qPCR assay to measure biomass and showed that two African maize lines, GAF4 and KDV1, had different fungal loads for the aflatoxigenic isolate (KSM014), fourteen days after infection. The qPCR assay revealed no significant variation in biomass between diseased and non-diseased maize tissues for GAF4, while KDV1 had a significantly higher biomass ( < 0.05) in infected shoots and roots compared to the control. The biocontrol strategy using an atoxigenic isolate (KSM012) against the toxigenic isolate (KSM014), showed aflatoxin production inhibition at the co-infection ratio, 50:50 for both maize lines (KDV1 > 99.7% and GAF ≥ 69.4%), as confirmed by bioanalytical techniques. As far as we are aware, this is the first report in Kenya where the biomass of from maize tissue was detected and quantified using a qPCR assay. Our results suggest that maize lines, which have adequate resistance to together with the appropriate biocontrol strategy, could limit outbreaks of aflatoxicoses.
玉米的定植会产生对人类和动物健康都有害的霉菌毒素。对感染有抗性的玉米株系进行筛选，并结合生物防治策略，有助于最大限度地减少后续黄曲霉毒素污染。我们开发了一种 qPCR 检测方法来测量生物量，并发现两种非洲玉米株系 GAF4 和 KDV1 在感染后的第 14 天对产黄曲霉的分离株（KSM014）具有不同的真菌负荷。qPCR 检测结果显示，对于 GAF4，在患病和未患病的玉米组织之间，生物量没有明显变化，而与对照相比，KDV1 感染的茎和根中的生物量明显更高（<0.05）。使用非产毒分离株（KSM012）对产毒分离株（KSM014）进行生物防治策略，在两种玉米株系（KDV1 > 99.7%和 GAF ≥ 69.4%）的共感染比例下显示出抑制黄曲霉毒素产生，这通过生物分析技术得到了证实。据我们所知，这是肯尼亚首次使用 qPCR 检测方法检测和定量玉米组织中黄曲霉的生物量。我们的研究结果表明，具有足够抗性和适当生物防治策略的玉米株系可以限制黄曲霉毒素病的爆发。
