塞尔维亚玉米田中产黄曲霉毒素的分布、遗传多样性和生物防治。
Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic in Serbian Maize Fields.
机构信息
Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
出版信息
Toxins (Basel). 2021 Sep 27;13(10):687. doi: 10.3390/toxins13100687.
Maize is one of the leading export products in the Republic of Serbia. As a country where economic development depends on agriculture, maize production plays a critical role as a crop of strategic importance. Potential aflatoxin contamination of maize poses a risk to food and feed safety and tremendous economic losses. No aflatoxin contamination of maize samples harvested in 2019 and 2020 in different localities in the Republic of Serbia was detected by the Enzyme-Linked Immunosorbent Assay (ELISA) test and High-Performance Liquid Chromatography (HPLC) method. On the other hand, the Cluster Amplification Patterns (CAP) analyses of the isolated strains from 2019 maize samples confirmed the presence of key biosynthesis genes responsible for aflatoxin production. Artificial inoculation and subsequent HPLC analysis of the inoculated maize samples confirmed the high capacity of the strains for aflatoxin production, pointing to a high risk of contamination under favorable conditions. Prevention of aflatoxin contamination is primarily based on control, where biocontrol agents play a significant role as sustainable disease management tools. In this study, antagonistic activity screening of the novel strains belonging to the genus indicated superior suppression of strains by two strains isolated from the rhizosphere of
玉米是塞尔维亚共和国的主要出口产品之一。作为一个经济发展依赖农业的国家，玉米生产作为一种具有战略重要性的作物，发挥着至关重要的作用。玉米中潜在的黄曲霉毒素污染对食品安全和饲料安全构成了威胁，并造成了巨大的经济损失。通过酶联免疫吸附试验（ELISA）和高效液相色谱法（HPLC）检测，在塞尔维亚共和国不同地区 2019 年和 2020 年收获的玉米样本中均未发现黄曲霉毒素污染。另一方面，对 2019 年玉米样本中分离出的菌株进行聚类扩增图谱（CAP）分析，证实了存在关键生物合成基因，这些基因负责黄曲霉毒素的产生。对接种玉米样本进行人工接种和后续 HPLC 分析，证实了这些菌株产生黄曲霉毒素的高能力，表明在有利条件下存在高污染风险。黄曲霉毒素污染的预防主要基于生物防治，其中生物防治剂作为可持续的疾病管理工具发挥着重要作用。在本研究中，对属于根霉属的新型菌株进行了拮抗活性筛选，结果表明，从根际分离出的两株根霉菌株对 菌株具有优越的抑制作用。
Zotero 插件