Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Erom Company Limited, R&D Center, 111, Toegye Nonggong-ro, Chuncheon-si, Gangwon-do 24427, Korea.
Int J Mol Sci. 2019 Mar 25;20(6):1496. doi: 10.3390/ijms20061496.
In this study, we combined enzymatic hydrolysis and lactic acid fermentation to generate an antihypertensive product. Soybean protein isolates were first hydrolyzed by Prozyme and subsequently fermented with EBD1. After fermentation, the in vitro angiotensin-converting enzyme (ACE) inhibitory activity of the product (P-SPI) increased from 60.8 ± 2.0% to 88.24 ± 3.2%, while captopril (a positive control) had an inhibitory activity of 94.20 ± 5.4%. Mass spectrometry revealed the presence of three potent and abundant ACE inhibitory peptides, PPNNNPASPSFSSSS, GPKALPII, and IIRCTGC in P-SPI. Hydrolyzing P-SPI with gastrointestinal proteases did not significantly affect its ACE inhibitory ability. Also, oral administration of P-SPI (200 mg/kg body weight) to spontaneous hypertensive rats (SHRs) for 6 weeks significantly lowered systolic blood pressure (-19 ± 4 mm Hg, < 0.05) and controlled body weight gain relative to control SHRs that were fed with physiological saline. Overall, P-SPI could be used as an antihypertensive functional food.
在这项研究中，我们结合酶解和乳酸发酵来生成一种具有降血压功能的产品。首先，大豆蛋白分离物被 Prozyme 水解，然后用 EBD1 发酵。发酵后，产物（P-SPI）的体外血管紧张素转换酶（ACE）抑制活性从 60.8 ± 2.0%增加到 88.24 ± 3.2%，而卡托普利（阳性对照）的抑制活性为 94.20 ± 5.4%。质谱分析表明，P-SPI 中存在三种具有强抑制活性的丰富 ACE 抑制肽，即 PPNNNPASPSFSSSS、GPKALPII 和 IIRCTGC。用胃肠蛋白酶水解 P-SPI 不会显著影响其 ACE 抑制能力。此外，给自发性高血压大鼠（SHRs）口服 P-SPI（200 mg/kg 体重）6 周，可显著降低收缩压（-19 ± 4mmHg， < 0.05），并控制体重增长，与给予生理盐水的对照 SHRs 相比。总的来说，P-SPI 可作为一种具有降血压功能的功能性食品。
