Food and Nutritional Sciences Program, Department of Family and Consumer Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA.
Int J Mol Sci. 2024 Jul 7;25(13):7463. doi: 10.3390/ijms25137463.
Hypertension is a major controllable risk factor associated with cardiovascular disease (CVD) and overall mortality worldwide. Most people with hypertension must take medications that are effective in blood pressure management but cause many side effects. Thus, it is important to explore safer antihypertensive alternatives to regulate blood pressure. In this study, peanut protein concentrate (PPC) was hydrolyzed with 3-5% Alcalase for 3-10 h. The in vitro angiotensin-converting enzyme (ACE) and renin-inhibitory activities of the resulting peanut protein hydrolysate (PPH) samples and their fractions of different molecular weight ranges were determined as two measures of their antihypertensive potentials. The results show that the crude PPH produced at 4% Alcalase for 6 h of hydrolysis had the highest ACE-inhibitory activity with IC being 5.45 mg/mL. The PPH samples produced with 3-5% Alcalase hydrolysis for 6-8 h also displayed substantial renin-inhibitory activities, which is a great advantage over the animal protein-derived bioactive peptides or hydrolysate. Remarkably higher ACE- and renin-inhibitory activities were observed in fractions smaller than 5 kDa with IC being 0.85 and 1.78 mg/mL. Hence, the PPH and its small molecular fraction produced under proper Alcalase hydrolysis conditions have great potential to serve as a cost-effective anti-hypertensive ingredient for blood pressure management.
高血压是全球范围内与心血管疾病（CVD）和总体死亡率相关的主要可控风险因素。大多数高血压患者必须服用能有效控制血压但会引起许多副作用的药物。因此，探索更安全的降压替代方法来调节血压非常重要。在这项研究中，花生蛋白浓缩物（PPC）用 3-5%的碱性蛋白酶水解 3-10 小时。测定所得花生蛋白水解产物（PPH）样品及其不同分子量范围的各部分的体外血管紧张素转换酶（ACE）和肾素抑制活性，作为其降压潜力的两个衡量标准。结果表明，在 4%碱性蛋白酶水解 6 小时产生的粗 PPH 具有最高的 ACE 抑制活性，IC 为 5.45mg/ml。用 3-5%碱性蛋白酶水解 6-8 小时产生的 PPH 样品也显示出很强的肾素抑制活性，这是优于动物蛋白来源的生物活性肽或水解产物的一个巨大优势。IC 为 0.85 和 1.78mg/ml 的小于 5kDa 的小分子量部分中观察到 ACE 和肾素抑制活性显著提高。因此，在适当的碱性蛋白酶水解条件下产生的 PPH 和其小分子量部分具有作为用于血压管理的经济有效的抗高血压成分的巨大潜力。
