Sakihama N, Shin M, Toda H
J Biochem. 1986 Jul;100(1):43-7. doi: 10.1093/oxfordjournals.jbchem.a121704.
Hydrophobic chromatography on a TSK-gel Phenyl-5PW column separated highly purified spinach ferredoxin into two distinct molecular species in their native forms. The two ferredoxins showed almost the same absorption spectra in spite of a difference in amino acid composition. Both ferredoxins were active in the NADPH-cytochrome c reducing system, and no significant difference was observed between their activities. The new separation method was also applied to ferredoxins highly purified from wheat plants and barley. Interestingly, all ferredoxin preparations so far examined contained two molecular species of ferredoxin.
在TSK-凝胶苯基-5PW柱上进行疏水层析，可将高度纯化的菠菜铁氧化还原蛋白分离成两种不同的天然分子形式。尽管氨基酸组成有所不同，但这两种铁氧化还原蛋白显示出几乎相同的吸收光谱。两种铁氧化还原蛋白在NADPH-细胞色素c还原系统中均具有活性，且它们的活性之间未观察到显著差异。这种新的分离方法也应用于从小麦植株和大麦中高度纯化的铁氧化还原蛋白。有趣的是，迄今为止所检测的所有铁氧化还原蛋白制剂都含有两种分子形式的铁氧化还原蛋白。
