Sakihama N, Shin M
Arch Biochem Biophys. 1987 Aug 1;256(2):430-4. doi: 10.1016/0003-9861(87)90599-6.
Crude ferredoxin preparations were obtained from blue-green algae, green algae, ferns, and higher plants. We analyzed the preparations by high-pressure liquid chromatography using two different types of columns, a hydrophobic phenyl-5PW column and an ion-exchange DEAE-5PW column. Two ferredoxins were detected in all plants analyzed. The ferredoxins from some plants were separated by use of both columns and those from others were separated by one of the two columns. Thus, there were three possible ways in which pairs of ferredoxins from a single species of plant could be separated. We suspect that there are two different ferredoxins in most if not all species of plants.
粗制铁氧化还原蛋白制剂取自蓝藻、绿藻、蕨类植物和高等植物。我们使用两种不同类型的色谱柱，即疏水苯基-5PW柱和离子交换DEAE-5PW柱，通过高压液相色谱法对这些制剂进行了分析。在所有分析的植物中均检测到两种铁氧化还原蛋白。一些植物的铁氧化还原蛋白可通过两种色谱柱进行分离，而另一些植物的铁氧化还原蛋白则可通过其中一种色谱柱进行分离。因此，来自单一植物物种的铁氧化还原蛋白对有三种可能的分离方式。我们怀疑，即使不是所有植物物种，大多数植物中也存在两种不同的铁氧化还原蛋白。
