用于生产结核分枝杆菌皮肤试验制剂的聚丙烯酰胺凝胶电泳分级分离方法的评估。I. 生产、理化特性及血清学分析。

An evaluation of the polyacrylamide gel electrophoresis fractionation method for the production of Mycobacterium tuberculosis skin test preparations. I. Production, physiochemical characterization and serological analyses.

作者信息

Affronti L F, Lind A, Ouchterlony O, Ridell M, Wisingerova E

出版信息

J Biol Stand. 1986 Jul;14(3):177-86. doi: 10.1016/0092-1157(86)90002-8.

Abstract

As a part of a cooperative inter-laboratory WHO supported project raw tuberculins were produced and purified protein derivative (PPD, 18.7 g protein) was prepared. Employing a multistage preparative polyacrylamide gel electrophoresis (PAGE) method the PPD was separated into four fractions corresponding to 15, 7, 4.75 and 3.5% gel concentrations. The PAGE procedure resulted in three lots of material--each representing 11 electrophoretic runs. Immunodiffusion analyses showed that the largest number of precipitinogens was found in the 15% fractions and that some precipitinogens cross-reacted with preparations of Mycobacterium bovis BCG, M. intracellulare, M. kansasii, M. smegmatis and M. vaccae.

摘要

作为世界卫生组织支持的合作性实验室间项目的一部分，制备了粗制结核菌素，并制备了纯化蛋白衍生物（PPD，18.7克蛋白质）。采用多阶段制备聚丙烯酰胺凝胶电泳（PAGE）方法，将PPD分离成四个组分，分别对应15%、7%、4.75%和3.5%的凝胶浓度。PAGE程序产生了三批材料，每批代表11次电泳运行。免疫扩散分析表明，15%组分中发现的沉淀原数量最多，并且一些沉淀原与牛分枝杆菌卡介苗、胞内分枝杆菌、堪萨斯分枝杆菌、耻垢分枝杆菌和母牛分枝杆菌的制剂发生交叉反应。