Thoen C O, Angus R D, Swanson M
Am J Vet Res. 1977 Jul;38(7):1019-22.
A method for the evaluation of Mycobacterium bovis purified protein derivative (PPD) tuberculin in experimentally infected cattle is presented. The development of skin test responses in M bovis-infected cattle was determined for International Standard PPD-S, M bovis PPD-2, and M bovis PPD-5 at 24, 48, and 72 hours. Significantly larger reactions (dermal thickness) were observed at 48 and 72 hours than at 24 hours (P = 0.001). Statistically significant differences were not detected in the responses obtained with M bovis PPD-2, M bovis PPD-5, and International Standard PPD-S if comparisons were made at approximately the same concentrations in M bovis-infected cattle (P greater than 0.25). In Mycobacterium avium-infected cattle, M bovis PPD-2 produced skin test responses that were significantly smaller than responses obtained using M avium PPD-2 (P = 0.001). Significant variation was not observed in the PPD-S responses in 2 groups of M bovis-infected cattle (P greater than 0.1).
本文介绍了一种在实验感染牛中评估牛分枝杆菌纯化蛋白衍生物（PPD）结核菌素的方法。测定了国际标准PPD-S、牛分枝杆菌PPD-2和牛分枝杆菌PPD-5在24、48和72小时时感染牛分枝杆菌的牛皮肤试验反应的发展情况。在48和72小时时观察到的反应（皮肤厚度）明显大于24小时时（P = 0.001）。如果在感染牛分枝杆菌的牛中以大致相同的浓度进行比较，在牛分枝杆菌PPD-2、牛分枝杆菌PPD-5和国际标准PPD-S获得的反应中未检测到统计学上的显著差异（P大于0.25）。在感染鸟分枝杆菌的牛中，牛分枝杆菌PPD-2产生的皮肤试验反应明显小于使用鸟分枝杆菌PPD-2获得的反应（P = 0.001）。在两组感染牛分枝杆菌的牛中，PPD-S反应未观察到显著差异（P大于0.1）。
