Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, Pl-87 100 Toruń, Poland.
Interdisciplinary Center for Modern Technologies, Nicolaus Copernicus University in Torun, Wileńska 4, 87⁻100 Toruń, Poland.
Int J Mol Sci. 2019 Mar 29;20(7):1588. doi: 10.3390/ijms20071588.
A method of purifying cyclododecyl 2,4-dihydroxybenzoate as a potential replacement template molecule for preparation of molecularly-imprinted polymers for isolation of zearalenone in urine was developed. Full physicochemical characteristics of cyclododecyl 2,4-dihydroxybenzoate for the first time included crystallographic analysis and molecular modelling, which made possible the determination of the similarity between the cyclododecyl 2,4-dihydroxybenzoate and zearalenone molecules. The obtained molecularly-imprinted polymers show very high in vitro selectivity towards zearalenone due to specific interactions (e.g., hydrogen bonding, molecular recognition interaction). The achieved extraction recovery exceeds 94% at the tested concentration levels (20⁻500 ng·mL) with a relative standard deviation below 2%. Immunosorbents were found to have lower recoveries (below 92.5%) and RSD value between 2 and 4% for higher concentrations of the studied substance (400 ng·mL).
建立了一种纯化环十二烷基 2,4-二羟基苯甲酸酯的方法，该化合物可作为潜在的替代模板分子，用于制备用于尿液中玉米赤霉烯酮分离的分子印迹聚合物。首次对环十二烷基 2,4-二羟基苯甲酸酯的全部物理化学特性进行了研究，包括晶体学分析和分子建模，这使得确定环十二烷基 2,4-二羟基苯甲酸酯和玉米赤霉烯酮分子之间的相似性成为可能。由于特异性相互作用（例如氢键、分子识别相互作用），所得的分子印迹聚合物对玉米赤霉烯酮表现出非常高的体外选择性。在测试浓度水平（20⁻500 ng·mL）下，提取回收率超过 94%，相对标准偏差低于 2%。对于研究物质的较高浓度（400 ng·mL），免疫吸附剂的回收率较低（低于 92.5%），且 RSD 值在 2%至 4%之间。
